As nanotechnology is still an emerging field with an increasing variety of engineered nanomaterials (ENMs), products and applications, there is a great relevance in assessing health and environmental hazards while exposure scenarios are essential tools.
Exposure studies are relevant regarding to various criteria like e.g. the duration which is the short-term (acute) exposure versus the long-term (chronic) exposure but also the way of studying by in vivo and in vitro tests. Especially for the exposure to ENMs, exposure scenarios are often divided into external and internal exposure. External exposure refers to the whole dose to which an organism is exposed by inhalation, skin contact or oral ingestion. Internal exposure is formed only to that fraction of the initial dose that is distributed or absorbed throughout the body via systemic circulation resulting in toxicokinetics and toxicodynamics.
For studying EMNs and their derivates in various exposure matrices, ICPMS is often applied as key analytical technique while different approaches like total quantification by ICPMS, single particle ICPMS or hyphenated to separation techniques like asymmetric flow field flow fractionation (AF4) are exploited.
This presentation focuses on relevant analytical approaches in the above described exposure routes by highlighting ICPMS methodology and the use of relevant complementary techniques.
H. Braakhuis, S.K. Kloet, S. Kezic, F. Kuper, M. Park, S. Bellmann, M. van der Zande, S. Le Gac, P. Krystek, R.J.B. Peters, I.M.C.M. Rietjens, H. Bouwmeester, Progress and future of in vitro models to study translocation of nanoparticles, Archives of Toxicology, 89, 1469-1495 (2015)
P. Krystek, M. Park, H. Braakhuis, W.H. de Jong, Applications of ICPMS in biodistribution studies of (engineered) nanoparticles, Encyclopedia of Analytical Chemistry, doi:10.1002/9870470027318.a9337 (2013)
M.R. Miller, J. Raftis, J.P. Langrish, S.G. McLean, P. Samutrtai, S.P. Connell, S. Wilson, A.T. Vesy, P.H.B. Fokkens, A.J.F. Boere, P. Krystek, C.J. Campbell, P.W.F. Hadoke, K. Donaldson, F.R. Cassee, D.E. Newby, R. Duffin and N.L. Mills, Inhaled nanoparticles accumulate at sites of vascular disease, ACS nano, 11, 4542-4552 (2017)
P. Krystek, M.J. Spallek, T. Klein, J de Boer, Asymmetric flow field flow fractionation analysis of engineered nanoparticles in mouthwashes and their interaction with saliva, LC GC North America, 34, 1-6 (2016)
C. Bianco, S. Kezic, M.J. Visser, O.A. Pluut, G. Adami, P. Krystek, Pilot study on the identification of silver in skin layers and urine after dermal exposure to a functionalized textile, Talanta, 136, 23-28 (2015)
P. Krystek, K. Kettler, B. van der Wagt, W.H. de Jong, Exploring influences on the cellular uptake of medium-sized silver nanoparticles into THP-1 cells, Microchemical Journal, 120, 45-50 (2015)
11 Jun 2018 → 18 Jun 2018
62nd International Conference on Analytical Sciences and Spectroscopy, Toronto, Canada