Raw data from: Natural alleles at the Doa locus underpin evolutionary changes in Drosophila lifespan and fecundity

Evolve and resequence' (E&amp;R) studies in <em>Drosophila melanogaster</em> have identified many candidate loci underlying the evolution of ageing and life history, but experiments that validate the effects of such candidates remain rare. In a recent E&amp;R study we have identified several alleles of the LAMMER kinase <em>Darkener of apricot</em> (<em>Doa</em>) as candidates for evolutionary changes in lifespan and fecundity. Here, we use two complementary approaches to confirm the functional role of <em>Doa</em> in life-history evolution. First, we used transgenic RNAi to study the effects of<em> Doa</em> at the whole-gene level. Ubiquitous silencing of expression in adult flies reduced both lifespan and fecundity, indicating pleiotropic effects. Second, to characterize segregating variation at <em>Doa</em>, we examined four candidate single nucleotide polymorphisms (SNPs;<em> Doa-1, -2, -3, -4</em>) using a genetic association approach. Three candidate SNPs had effects that were qualitatively consistent with expectations based on our E&amp;R study: <em>Doa</em>-2 pleiotropically affected both lifespan and late-life fecundity; <em>Doa</em>-1 affected lifespan (but not fecundity), and <em>Doa</em>-4 affected late-life fecundity (but not lifespan). Finally, the last candidate allele (<em>Doa</em>-3) also affected lifespan, but in the opposite direction than predicted.