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Dr. Jeroen Kool is an analytical chemist with research interests in high resolution screening of biologically active compounds. During his PhD study (2001-2005) at VU University, he focused on the integration of chemical - e.g. mass spectrometry (MS) - with biochemical detection after separation methodologies. Moreover, he worked on post-column coupling of enzyme and receptor assays that are relevant for toxicological and pharmacological studies to study drug-drug interactions, reactive intermediates and pharmacologically active metabolites. Following his doctorate, Kool joined Kiadis Pharma where he was responsible for target evaluation, hit screening and identification and lead optimization processes. His work mainly involved high resolution screening technologies in combination with MS identification, and the mammalian cellular screening portfolio.

 

Dr. Kool continued his academic career as a postdoctoral fellow in the Biomolecular Mass Spectrometry group at Utrecht University. There he worked on proteomics (biomarker discovery) focusing on complex regional pain syndrome. In 2007, Dr. Kool was appointed assistant professor at the Division of Biomolecular Analysis at VU University, where he is responsible for the research line Bioactivity Screening Methodologies. His research achievements allow full compatibility of analytical separations with biological assays (including cellular) and parallel MS detection for investigation of bioactive mixtures (metabolic mixtures, venoms, natural extracts) using miniaturized setups and nanospotting technologies.

 

In Dr. Kool’s focus on identification and characterization of bioactive compounds in biological mixtures, hyphenated analytics combining mass spectrometry and chromatography with novel bioassay techniques are integrated in one platform. We hyphenate both LC and GC separations to bioassays for identification of biologically active toxicants in food and the environment. In case of GC fractionations, we developed and patented an automated system for high resolution fractionation of complete GC chromatograms with parallel chemical detection, such as FID and MS. With this new technology one of our visions is to develop methods for screening organisms known to produce volatile antibiotics. Identification of these antibiotics might result in developing them further as inhaler administration type of drug candidates for pneumonia infections by bacteria such as tuberculosis and legionella.

 

Also, we developed analytical methodologies for bioactivity profiling of metabolic mixtures from drugs and lead compounds targeting GPCRs, nuclear receptors, protein kinases and ion channels. Currently, we are working on post-column microfluidics and nanofractionation analytics for analysis of bioactive mixtures only available in low amounts. Nice examples are insect and animal venoms that contain many different, highly potent, and sometimes very selective protein ligands for a large variety of medicinal targets. These analytics have additional implementation of proteomics approaches for identification of venom peptides for venom based drug discovery programs towards novel biopharmaceutical candidates.

 

The latest research Dr. Kool has initiated is directed at microfluidic spotting techniques (using nanospotters) for combining nanoLC with post-column array spotted bioassays. Readout of biological responses will occur with our new confocal microscope imaging scanner currently being developed together with our university’s precision mechanics and instrumental development groups. The microfluidics spotter & confocal imaging scanner technology will be directed at the discovery and identification of new antibiotic drug candidates in complex natural extracts from tropical plants and from coral reef anemones, sponges, and other invertebrates.

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Research Output 2000 2018

  • 81 Article
  • 5 PhD Thesis - Research VU, graduation VU
  • 1 Review article
Trapped ions
Spectrometry
Molecular structure
Conformations
Ions

Advanced bioactivity screening analytics for rapid identification of environmental toxicants

Jonker, L. W. 2018 217 p.

Research output: ThesisPhD Thesis - Research VU, graduation VU

Open Access
File

Angular scanning and variable wavelength surface plasmon resonance allowing free sensor surface selection for optimum material- and bio-sensing

Lakayan, D., Tuppurainen, J., Albers, M., van Lint, M. J., van Iperen, D. J., Weda, J. J. A., Kuncova-Kallio, J., Somsen, G. W. & Kool, J. 15 Apr 2018 In : Sensors and Actuators, B: Chemical. 259, 15 April, p. 972-979 8 p.

Research output: Contribution to journalArticle

Surface plasmon resonance
surface plasmon resonance
Scanning
Wavelength
scanning

Development of a surface plasmon resonance sensor for coupling to capillary electrophoresis allowing affinity assessment of protein mixture components

Domínguez-Vega, E., Haselberg, R., Iperen, D. V., Kool, J., Somsen, G. W. & de Jong, G. J. 1 Jan 2018 In : Sensors and Actuators B-Chemical. 254, p. 1040-1047 8 p.

Research output: Contribution to journalArticle

Capillary electrophoresis
Surface plasmon resonance
electrophoresis
surface plasmon resonance
affinity
Endocrine Disruptors
endocrine disruptor
Fractionation
Surface waters
Assays