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Personal profile

Personal information

I am an all-round analytical chemist with a strong background in capillary electrophoresis and liquid chromatography hyphenated with mass spectrometry. I use these technologies to characterize intact proteins. This ranges from proteins as small as insulin up to large protein complexes. I am interested in determining protein heterogeneity, getting insights in degradation processes, mapping intentional modifications and studying protein-protein interactions. Recently, my first personal research project was granted in which I will also focus on the bioanalysis of proteins.

In all this research the goal is to keep the protein intact from sample to detector, as this reduces data complexity and minimizes risk of introducing artefacts along the way. As studying these properties of intact proteins is not straightforward, I develop and apply the required advanced hyphenated analytics. All of this is done in the context of biopharmaceutical, clinical, and doping analysis, often in collaboration with companies and other university groups. 

Ancillary activities

  • Capillary electrophoresis user group | Amsterdam | Bestuurder | 2015-01-01
  • Discussion group Separation methods for Polymers | Amsterdam | Board member | 2016-08-01
  • F. Hoffman la Roche | Basel | Adviseur | 2018-04-01

Ancillary activities are updated daily

Education/Academic qualification

Analytical Chemistry, PhD, Utrecht University

Apr 2006Jun 2010

Analytical Sciences, Master, Vrije Universiteit Amsterdam

Sep 2004Apr 2006

Applied Analytical Chemistry , Bachelor, Saxion University of Applied Sciences, Handelskade 75, 7417 DH Deventer

Sep 2000Sep 2004

Keywords

  • QD Chemistry
  • intact protein analsysis
  • liquid chromatography
  • capillary electrophoresis
  • mass spectrometry
  • hyphenation
  • proteoform characterization
  • biopharmaceuticals

Fingerprint Fingerprint is based on mining the text of the person's scientific documents to create an index of weighted terms, which defines the key subjects of each individual researcher.

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Proteins Chemical Compounds
Single-Domain Antibodies Chemical Compounds
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Pharmaceutical Preparations Chemical Compounds

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Research Output 2006 2018

  • 28 Article
  • 2 Chapter
  • 2 Review article
  • 1 Conference contribution

Capillary Electrophoresis: Trends and Recent Advances

Voeten, R. L. C., Ventouri, I. K., Haselberg, R. & Somsen, G. W., 6 Feb 2018, In : Analytical Chemistry. 90, 3, p. 1464-1481 18 p.

Research output: Contribution to JournalReview articleAcademicpeer-review

Development of a capillary zone electrophoresis method to quantify E. coli L-asparaginase and its acidic variants

Yao, H., Vandenbossche, J., Sänger-van de Griend, C. E., Janssens, Y., Fernández, C. S., Xu, X., Wynendaele, E., Somsen, G. W., Haselberg, R. & De Spiegeleer, B., 15 May 2018, In : Talanta. 182, p. 83-91 9 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Asparaginase
Capillary Electrophoresis
Electrophoresis
Escherichia coli
Electrolytes

Development of a surface plasmon resonance sensor for coupling to capillary electrophoresis allowing affinity assessment of protein mixture components

Domínguez-Vega, E., Haselberg, R., Iperen, D. V., Kool, J., Somsen, G. W. & de Jong, G. J., 1 Jan 2018, In : Sensors and Actuators B-Chemical. 254, p. 1040-1047 8 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Capillary electrophoresis
Surface plasmon resonance
electrophoresis
surface plasmon resonance
affinity

Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-flow sheathless capillary electrophoresis-mass spectrometry

Haselberg, R., De Vijlder, T., Heukers, R., Smit, M. J., Romijn, E. P., Somsen, G. W. & Domínguez-Vega, E., 1 Jan 2018, (Accepted/In press) In : Analytica Chimica Acta.

Research output: Contribution to JournalArticleAcademicpeer-review

Capillary electrophoresis
Capillary Electrophoresis
low flow
antibody
Mass spectrometry

High-resolution glycoform profiling of intact therapeutic proteins by hydrophilic interaction chromatography-mass spectrometry

Domínguez-Vega, E., Tengattini, S., Peintner, C., van Angeren, J., Temporini, C., Haselberg, R., Massolini, G. & Somsen, G. W., Jul 2018, In : Talanta. 184, July, p. 375-381 7 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Chromatography
Hydrophobic and Hydrophilic Interactions
Mass spectrometry
Mass Spectrometry
Liquid chromatography