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Personal profile

Research

Bacterial respiration and bioenergetics

Denitrification and nitrous oxide emissions

Regulation and adaptive responses of bacterial regulatory networks

Bioenergetics of Mycobacteria; in search of novel drug targets

Microbial physiology

Systems Ecology

Bioremediation of contaminated soils; dioxins, oil and farmaceuticals

Microbial communities organization and function

Personal information

Short description of research experience and interests

Rob van Spanning is  Assistant Professor in the Department of Molecular Cell Biology at the Vrije Universiteit of  Amsterdam, The Netherlands. He holds a PhD in microbial physiology and molecular microbiology from the same university. His research interests are i) in understanding versatility and adaptive responses of bacterial respiration (from gene to function and back), and ii) in a fundamental understanding of make up and activity of microbial communities and their succession upon changes in their environment. For i) he studies the genetic and metabolic regulation, cellular organisation, function and biochemistry of a range of aerobic and anaerobic electron transport systems. He has ample experience in cloning, (homologous and heterologous) expression and in functional analyses of bacterial redox enzymes. For ii) he correlates microbial community make up to their function using different types of metagenomics analyses. He is skilled in i) a variety of genetic and analytical methods concerned with the functional characterization of enzymes (spectroscopy, reporter gene fusions, redox titrations, quinone/quinol extractions, kinetics of substrate consumption/product formation, purification, up to date general genetic and biochemical procedures), in ii) a variety of culturing techniques (batch, fed batch, chemostats, auxostats, retentostats), and in iii) fluorescence spectroscopy (fluorimeters, confocal microscopy).

Ancillary activities

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Keywords

  • Microbial ecology
  • Metagenomics
  • Community profiling
  • Bioremediation
  • Bacterial fingerprinting
  • Mesocosms
  • Bacterial respiration
  • Denitrification
  • Nitrous oxide emissions
  • Greenhouse gases
  • Free energy transduction
  • Nitrification

Fingerprint Fingerprint is based on mining the text of the person's scientific documents to create an index of weighted terms, which defines the key subjects of each individual researcher.

Paracoccus denitrificans Medicine & Life Sciences
Genes Medicine & Life Sciences
Nitrite Reductases Medicine & Life Sciences
Oxidoreductases Medicine & Life Sciences
Nitrites Medicine & Life Sciences
Denitrification Medicine & Life Sciences
Multigene Family Medicine & Life Sciences
Growth Medicine & Life Sciences

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Research Output 1986 2018

Comparison of landfarming amendments to improve bioremediation of petroleum hydrocarbons in Niger Delta soils

Brown, D. M., Okoro, S., van Gils, J., van Spanning, R., Bonte, M., Hutchings, T., Linden, O., Egbuche, U., Bruun, K. B. & Smith, J. W. N., 15 Oct 2017, In : Science of the Total Environment. 596-597, p. 284-292 9 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Bioremediation
Petroleum
petroleum hydrocarbon
Hydrocarbons
bioremediation

Sulfur cave (Romania), an extreme environment with microbial mats in a Co2-H2S/O2 gas chemocline dominated by mycobacteria

Sarbu, S. M., Aerts, J. W., Flot, J. F., van Spanning, R. J. M., Baciu, C., Ionescu, A., Kis, B. M., Incze, R., Siko-Barabasi, S., Para, Z., Hegyeli, B., Atudorei, N. V., Barr, C., Nealson, K. H., Forray, F. L., Lascu, C., Fleming, E. J., Bitter, W. & Popa, R., 1 Jan 2018, In : International Journal of Speleology. 47, 2, p. 173-187 15 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
microbial mat
cave
sulfur
gas
biofilm

Molecular genetics of the genus Paracoccus: metabolically versatile bacteria with bioenergetic flexibility

Baker, S. C., Ferguson, S. J., Ludwig, B., Page, M. D., Richter, O-MH. & van Spanning, R. J., Dec 1998, In : Microbiology and Molecular Biology Reviews. 62, 4, p. 1046-78 33 p.

Research output: Contribution to JournalReview articleAcademicpeer-review

Paracoccus
Paracoccus denitrificans
Energy Metabolism
Molecular Biology
Electron Transport Complex IV

The cytochrome bd-type quinol oxidase is important for survival of Mycobacterium smegmatis under peroxide and antibiotic-induced stress.

Lu, P., Heineke, M. H., Koul, A., Andries, K., Cook, G. M., Lill, H., van Spanning, R. J. M. & Bald, D., 2015, In : Scientific Reports. 5, 10333

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Mycobacterium smegmatis
Peroxides
Cytochromes
Anti-Bacterial Agents
bedaquiline

The heme-copper oxidase family consists of three distinct types of terminal oxidases and is related to nitric oxide reductase

van der Oost, J., de Boer, A. P., de Gier, J-W., Zumft, W. G., Stouthamer, A. H. & van Spanning, R. J., 1 Aug 1994, In : FEMS Microbiology Letters. 121, 1, p. 1-9 9 p.

Research output: Contribution to JournalReview articleAcademicpeer-review

Electron Transport Complex IV
Heme
Oxidoreductases
Denitrification
Cytochromes