If you made any changes in Pure these will be visible here soon.

Personal profile

Ancillary activities

No ancillary activities

Ancillary activities are updated daily

Personal information

I obtained my double-doctorate on muscle physiology in 2009 from the Manchester Metropolitan University and the VU University Amsterdam, in which I studied why smokers are more prone to fatigue. From 2009-2012, I worked at the University of Leeds (UK) on the control of mitochondrial respiration during exercise. After returning to the Netherlands, I worked on various projects related to cardiac metabolism and mitochondrial function at the Amsterdam UMC, locations VUmc and AMC. Since 2019, I am assistant-professor at the department of Human Movement Sciences of the VU University. I am involved in teaching and research in the areas of clinical exercise physiology, cellular physiology and mitochondrial function. Recently, I am involved in a large ESA/NASA-funded bedrest-study where the effects of microgravity are studied on the cellular and molecular level. I received different grants from The Physiological Society, Dutch Heart foundation, Amsterdam Cardiovascular Research and Amsterdam Movement Sciences.


General interests

My main research interest lies in the regulation of cardiac and skeletal muscle metabolism and mitochondrial function. I am interested in the fundamental aspects of mitochondrial bio-energetics as well as its consequence for whole cell and organ function. To study this, I use research methods ranging from imaging and spectroscopy, fluorescence microscopy and cellular and molecular techniques.



I have contributed to the understanding of how the skeletal muscle cell is able to adapt mitochondrial ATP production to demand. For this, I investigate muscle oxygen uptake under various conditions. Because of the link between mitochondrial dysfunction and exercise intolerance, and cardiac dysfunction, I have contributed to further understanding the consequences of mitochondrial dysfunction to functional outcomes in different conditions and chronic diseases. Some examples include the understanding of fatigue in current smokers and COPD patients, metabolic dysfunction in patients with right ventricular heart failure and cardiac dysfunction in patients with diabetes.

Besides, I have contributed to the development of various methodologies to assess metabolic and mitochondrial pathways from the whole-body level (including magnetic resonance imaging), via mitochondrial respirometry down to the molecular level (including live cell imaging).

Because of my time abroad, I have gathered a large network of collaborating scientists across the planet, including Japan, USA, Canada, Sweden, UK, Italy, Germany and Switzerland. This can provide ample opportunities for students to find internships abroad.


Current Research

My current research involves different projects in the area of 1) Muscle atrophy and metabolic alterations after inactivity (in collaboration with ESA/NASA), 2) Exercise capacity in various patient populations, 3) Development of new techniques assessing fundamental mitochondrial function in skeletal and cardiac muscle, and 4) Effects of inflammation on cardiac and skeletal muscle function.


Currently, I am teaching in the following courses:

Exercise and Clinical Immunology (Research Master Human Movement Sciences)

Applied Exercise Physiology

Hormones and Homeostasis (Amsterdam University College)


Besides, I supervise students during their internships and literature review projects.

Education/Academic qualification


  • QP Physiology
  • mitochondria
  • bioenergetics
  • muscle
  • metabolism
  • cardiac
  • Exercise Physiology
  • Exercise Endocrinology
  • hormones

Fingerprint Dive into the research topics where Rob Wust is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 2 Similar Profiles
Skeletal Muscle Medicine & Life Sciences
Muscles Medicine & Life Sciences
Cardiac Myocytes Medicine & Life Sciences
Muscle Fatigue Medicine & Life Sciences
Succinate Dehydrogenase Medicine & Life Sciences
Heart Failure Medicine & Life Sciences
Exercise Medicine & Life Sciences
Fatigue Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output 2007 2020

An iterative sparse deconvolution method for simultaneous multicolor 19 F-MRI of multiple contrast agents

Schoormans, J., Calcagno, C., Daal, M. R. R., Wüst, R. C. I., Faries, C., Maier, A., Teunissen, A. J. P., Naidu, S., Sanchez-Gaytan, B. L., Nederveen, A. J., Fayad, Z. A., Mulder, W. J. M., Coolen, B. F. & Strijkers, G. J., Jan 2020, In : Magnetic Resonance in Medicine. 83, 1, p. 228-239 12 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Contrast Media
Cell Tracking

Altered mitochondrial metabolism in the insulin resistant heart

Makrecka-Kuka, M., Liepinsh, E., Murray, A. J., Lemieux, H., Dambrova, M., Tepp, K., Puurand, M., Käämbre, T., Han, W. H., de Goede, P., O'Brien, K. A., Turan, B., Tuncay, E., Olgar, Y., Rolo, A. P., Palmeira, C. M., Boardman, N. T., Wüst, R. C. & Larsen, T. S., 16 Dec 2019, In : Acta Physiologica. p. e13430

Research output: Contribution to JournalReview articleAcademicpeer-review

Fatty Acids
Diabetic Cardiomyopathies
Oxidative Phosphorylation
Type 2 Diabetes Mellitus

Disturbed cardiac mitochondrial and cytosolic calcium handling in a metabolic risk-related rat model of heart failure with preserved ejection fraction

Miranda-Silva, D., Wüst, R. C. I., Conceição, G., Gonçalves-Rodrigues, P., Gonçalves, N., Gonçalves, A., Kuster, D. W. D., Leite-Moreira, A. F., van der Velden, J., de Sousa Beleza, J. M., Magalhães, J., Stienen, G. J. M. & Falcão-Pires, I., 13 Sep 2019, In : Acta Physiologica. p. e13378

Research output: Contribution to JournalArticleAcademicpeer-review

Heart Failure
Mitochondrial Swelling
Cardiac Myocytes

Emerging Magnetic Resonance Imaging Techniques for Atherosclerosis Imaging

Wüst, R. C. I., Calcagno, C., Daal, M. R. R., Nederveen, A. J., Coolen, B. F. & Strijkers, G. J., May 2019, In : Arteriosclerosis, Thrombosis, and Vascular Biology. 39, 5, p. 841-849 9 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Magnetic Resonance Imaging
Cardiovascular Diseases

Mitochondrial Dysfunction Underlies Cardiomyocyte Remodeling in Experimental and Clinical Atrial Fibrillation

Wiersma, M., van Marion, D. M. S., Wüst, R. C. I., Houtkooper, R. H., Zhang, D., Groot, N. M. S. D., Henning, R. H. & Brundel, B. J. J. M., 5 Oct 2019, In : Cells. 8, 10, 1202.

Research output: Contribution to JournalArticleAcademicpeer-review

Cardiac Myocytes
Atrial Fibrillation
Adenosine Triphosphate
Atrial Remodeling