prof. dr. Wouter Roos

prof. dr.

20032020
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Research Output 2003 2020

2020

DNA, proteins, membranes: exploring the physics behind biomolecular processes

Marchetti, M., 2020, 195 p.

Research output: PhD ThesisPhD Thesis - Research VU, graduation VUAcademic

Open Access
File
2019

Maturation of adenovirus primes the protein nano-shell for successful endosomal escape

Denning, D., Bennett, S., Mullen, T., Moyer, C., Vorselen, D., Wuite, G. J. L., Nemerow, G. & Roos, W. H., 7 Mar 2019, In : Nanoscale. 11, 9, p. 3799-3803 5 p.

Research output: Contribution to JournalArticleAcademicpeer-review

DNA
Genes
Fatigue of materials
Proteins
Medical nanotechnology

Real-Time Assembly of Viruslike Nucleocapsids Elucidated at the Single-Particle Level

Marchetti, M., Kamsma, D., Cazares Vargas, E., Hernandez García, A., Van Der Schoot, P., De Vries, R., Wuite, G. J. L. & Roos, W. H., 14 Aug 2019, In : Nano Letters. 19, 8, p. 5746-5753 8 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Nucleocapsid
assembly
Oligomers
Packaging
DNA
2018

Acetylation and phosphorylation of human TFAM regulate TFAM-DNA interactions via contrasting mechanisms

Shabestari, M. H., King, G. A., Taris, K-K. H., Pandey, A. K., Venkatesh, S., Thilagavathi, J., Singh, K., Koppisetti, R. K., Temiakov, D., Roos, W. H., Suzuki, C. K. & Wuite, G. J. L., 20 Apr 2018, In : Nucleic Acids Research. 46, 7, p. 3633-3642 10 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access

A single point mutation in precursor protein VI doubles the mechanical strength of human adenovirus

van Rosmalen, M. G. M., Nemerow, G. R., Wuite, G. J. L. & Roos, W. H., Jun 2018, In : Journal of Biological Physics. 44, 2, p. 119-132 14 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
adenoviruses
Human Adenoviruses
Protein Precursors
Capsid
mutations

Atomic force microscopy-based mechanobiology

Krieg, M., Fläschner, G., Alsteens, D., Gaub, B. M., Roos, W. H., Wuite, G. J. L., Gaub, H. E., Gerber, C., Dufrêne, Y. F. & Müller, D. J., 1 Nov 2018, In : Nature Reviews Physics. 1, January, p. 41-57 17 p.

Research output: Contribution to JournalArticleAcademicpeer-review

biophysics
atomic force microscopy
mechanical properties
microscopy
fluorescence emission spectroscopy

Effect of dsDNA on the Assembly Pathway and Mechanical Strength of SV40 VP1 Virus-like Particles

van Rosmalen, M. G. M., Li, C., Zlotnick, A., Wuite, G. J. L. & Roos, W. H., 6 Nov 2018, In : Biophysical Journal. 115, 9, p. 1656-1665 10 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Simian virus 40
Virion
DNA Viruses
DNA
Buffers

Multilamellar nanovesicles show distinct mechanical properties depending on their degree of lamellarity

Vorselen, D., Marchetti, M., López-Iglesias, C., Peters, P. J., Roos, W. H. & Wuite, G. J. L., 21 Mar 2018, In : Nanoscale. 10, 11, p. 5318-5324 7 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Atomic force microscopy
Mechanical properties
Unilamellar Liposomes
Lipid bilayers
Nanoindentation

Nanomechanics of Extracellular Vesicles Reveals Vesiculation Pathways

Sorkin, R., Huisjes, R., Bošković, F., Vorselen, D., Pignatelli, S., Ofir-Birin, Y., Freitas Leal, J. K., Schiller, J., Mullick, D., Roos, W. H., Bosman, G., Regev-Rudzki, N., Schiffelers, R. M. & Wuite, G. J. L., 27 Sep 2018, In : Small. 14, 39, p. 1-8 8 p., 1801650.

Research output: Contribution to JournalArticleAcademicpeer-review

Nanomechanics
Lipids
Proteins
Blood
Erythrocytes

Probing cellular mechanics with acoustic force spectroscopy

Sorkin, R., Bergamaschi, G., Kamsma, D., Brand, G., Dekel, E., Ofir-Birin, Y., Rudik, A., Gironella, M., Ritort, F., Regev-Rudzki, N., Roos, W. H. & Wuite, G. J. L., 8 Aug 2018, In : Molecular Biology of the Cell. 29, 16, p. 2005-2011 7 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Mechanics
Acoustics
Spectrum Analysis
Erythrocytes
Biophysics

The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis

Vorselen, D., van Dommelen, S. M., Sorkin, R., Piontek, M. C., Schiller, J., Döpp, S. T., Kooijmans, S. A. A., van Oirschot, B. A., Versluijs, B. A., Bierings, M. B., van Wijk, R., Schiffelers, R. M., Wuite, G. J. L. & Roos, W. H., 23 Nov 2018, In : Nature Communications. 9, p. 1-9 9 p., 4960.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Hereditary Spherocytosis
erythrocytes
Mechanics
Blood
Erythrocytes

The Soft Side of Extracellular Vesicles

Sorkin, R., Huisjes, R., Boskovic, F., Vorselen, D., Pignatelli, S., Ofir-Birin, Y., Leal, J. K. F., Schiller, J., Roos, W. H., Bosman, G., Regev-Rudzki, N., Schiffelers, R. M. & Wuite, G. J. L., 2 Feb 2018, In : Biophysical Journal. 114, 3, p. 562A-562A

Research output: Contribution to JournalMeeting AbstractAcademic

Viruses under the loop: Their assembly process and capsid stiffness

van Rosmalen, M. G. M., 2018, 198 p.

Research output: PhD ThesisPhD Thesis - Research VU, graduation VUAcademic

Open Access
File
2017

Competition between Bending and Internal Pressure Governs the Mechanics of Fluid Nanovesicles

Vorselen, D., MacKintosh, F. C., Roos, W. H. & Wuite, G. J. L., Mar 2017, In : ACS Nano. 11, 3, p. 2628-2636

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access

Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling

Liang, Y., van der Valk, R. A., Dame, R. T., Roos, W. H. & Wuite, G. J. L., 10 Nov 2017, In : Scientific Reports. 7, 15275.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access

Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy

Hashemi Shabestari, M., Meijering, A. E. C., Roos, W. H., Wuite, G. J. L. & Peterman, E., 2017, Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods. Elsevier, Vol. 582. p. 85-119

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Optical tweezers
Fluorescence microscopy
Molecules
Biomolecules
Biological systems

Understanding Adenovirus maturation: A nanomechanics approach

Denning, D., Bennett, S., Mullen, T., Moyer, C., Wuite, G. J., Nemerow, G. & Roos, W. H., Jul 2017, In : European Biophysics Journal. 46, p. S184-S184 1 p., Supplement 1.

Research output: Contribution to JournalMeeting AbstractAcademic

Vertex-Specific Proteins pUL17 and pUL25 Mechanically Reinforce Herpes Simplex Virus Capsids

Snijder, J., Radtke, K., Anderson, F., Scholtes, L., Corradini, E., Baines, J., Heck, A. J. R., Wuite, G. J. L., Sodeik, B. & Roos, W. H., Jun 2017, In : Journal of Virology. 91, 12, UNSP e00123-17.

Research output: Contribution to JournalArticleAcademicpeer-review

2016

A Combined Imaging and Force Spectroscopy Approach Reveals the Material Properties of Viral Nanoparticles

Denning, D., Wuite, G. J. L. & Roos, W. H., 2016, In : Biophysical Journal. 110, 3, p. 500A-500A

Research output: Contribution to JournalMeeting AbstractOther research output

Open Access

Assembly and Mechanical Properties of the Cargo-Free and Cargo Loaded Bacterial Nanocompartment Encapsulin

Snijder, J., Kononova, O., Barbu, I. M., Uetrecht, C., Rurup, W. F., Koay, M. S. T., Cornelissen, J. J. L. M., Roos, W. H., Barsegov, V., Wuite, G. J. L. & Heck, A. J. R., 2016, In : Biomacromolecules. 17, 8, p. 2522-2529

Research output: Contribution to JournalArticleAcademicpeer-review

Viruses
Mechanical properties
Enzymes
Proteins
Molecular modeling
Open Access
Atomic Force Microscopy
Virion
Virology
Capsid
Observation

Controlled tip wear on high roughness surfaces yields gradual broadening and rounding of cantilever tips

Vorselen, D., Kooreman, E. S., Wuite, G. J. L. & Roos, W. H., 11 Nov 2016, In : Scientific Reports. 6, 36972.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access

Detection and Mechanical Characterization of Small Multilamellar Vesicles using Atomic Force Microscopy

Marchetti, M., Vorselen, D., Roos, W. H. & Wuite, G. J. L., 2016, In : Biophysical Journal. 110, 3, p. 73A-73A-73A

Research output: Contribution to JournalMeeting AbstractOther research output

Open Access

Fluctuating Nonlinear Spring Model of Mechanical Deformation of Biological Particles

Kononova, O., Snijder, J., Kholodov, Y., Marx, K. A., Wuite, G. J. L., Roos, W. H. & Barsegov, V., 2016, In : PLoS Computational Biology. 12, 1, e1004729.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Nonlinear Dynamics
Nanoparticles
Capsid
nanoparticles
capsid

Mechanical Unfolding of an Autotransporter Passenger Protein Reveals the Secretion Starting Point and Processive Transport Intermediates

Baclayon, M., van Ulsen, P., Mouhib, H., Hashemi Shabestari, M., Verzijden, T., Abeln, S., Roos, W. H. & Wuite, G. J. L., 2016, In : ACS Nano. 10, 6, p. 5710-5719

Research output: Contribution to JournalArticleAcademicpeer-review

protease
secretions
passengers
Hemoglobin
hemoglobin

Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM

Heinze, K., Sasaki, E., King, N. P., Baker, D., Hilvert, D., Wuite, G. J. L. & Roos, W. H., 2016, In : Journal of Physical Chemistry B. 120, 26, p. 5945-5952

Research output: Contribution to JournalArticleAcademicpeer-review

Nanoindentation
Mechanics
atomic force microscopy
proteins
Proteins
Open Access

Vesicle Mechanics: A Push into the Nanoscale

Vorselen, D., 2016, 188 p.

Research output: PhD ThesisPhD Thesis - Research VU, graduation VUAcademic

Open Access
File
2015

Building an artificial actin cortex on microscopic pillar arrays

Ayadi, R. & Roos, W. H., 2015, In : Methods in Cell Biology. 128, p. 105-124

Research output: Contribution to JournalArticleAcademicpeer-review

Actins
Artificial Cells
Biomimetics
Biopolymers
Eukaryotic Cells

DNA-Induced Viral Assembly Studied in Real-Time by Optical Tweezers, Acoustic Force Spectroscopy and Atomic Force Microscopy

van Rosmalen, M. G. M., Biebricher, A. S., Kamsma, D., Zlotnick, A., Wuite, G. J. L. & Roos, W. H., 2015, In : Biophysical Journal. 108, 2, p. 170A-170A

Research output: Contribution to JournalMeeting AbstractOther research output

Material properties of viral nanocages explored by atomic force microscopy

van Rosmalen, M. G. M., Roos, W. H. & Wuite, G. J. L., 2015, In : Methods in Molecular Biology. 1252, p. 115-137

Research output: Contribution to JournalArticleAcademicpeer-review

Atomic Force Microscopy
Capsid
Product Packaging
Diagnostic Imaging
Life Cycle Stages

Mechanics of Extracellular Vesicles from Red Blood Cells

Vorselen, D., Dommelen, S. M., van Loon, J. J. W. A., Schiffelers, R. M., Roos, W. H. & Wuite, G. J. L., 2015, In : Biophysical Journal. 108, 2, p. 242A-242A

Research output: Contribution to JournalMeeting AbstractOther research output

Open Access
2014

Elasticity of Biomolecules: probing, pushing and pulling using atomic force microscopy

Baclayon, M., 2014, 180 p.

Research output: PhD ThesisPhD Thesis - Research external, graduation VUAcademic

Open Access
File

In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription

Farge, G. A., Mehmedovic, M., Baclayon, M., Wildenberg, S. M. J. L., Roos, W. H., Gustafsson, C. M., Wuite, G. J. L. & Falkenberg, M., 2014, In : Cell Reports. 2014, 8, p. 66-74

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Transcription
DNA Replication
Mitochondrial DNA
DNA
Nucleic Acid Denaturation

Probing the Unbinding Kinetics of DNA-H-NS-DNA Protein Complexes by a High-Speed and High-Throughput Single-Molecule Pulling Assay using Atomic Force Microscopy

Liang, Y., Baclayon, M., van der Valk, R., Dame, R. T., Roos, W. H. & Wuite, G. J. L., 2014, In : Biophysical Journal. 106, 2, p. 386A-386A

Research output: Contribution to JournalMeeting AbstractOther research output

Open Access

Self-assembly and characterization of small and monodisperse dye nanospheres in a protein cage

Luque, D., Snijder, J., Brasch, M., Burnley, R. J., Koay, M. S. T., Carrascosa, J. L., Wuite, G. J. L., Roos, W. H., Heck, A. J. R., Cornelissen, J. L. M., Torres, C. & Caston, J. R., 2014, In : Chemical Science. 5, p. 575-581

Research output: Contribution to JournalArticleAcademicpeer-review

Nanospheres
Self assembly
Coloring Agents
Viruses
Proteins

The role of the cytoskeleton in sensing changes in gravity by nonspecialized cells

Vorselen, D., Roos, W. H., MacKintosh, F. C., Wuite, G. J. L. & van Loon, J. J. W. A., 2014, In : FASEB Journal. 28, 2, p. 536-547

Research output: Contribution to JournalArticleAcademicpeer-review

Gravitation
Cytoskeleton
Space Flight
Altered Gravity
Biophysics
2013

Integrin and defensin modulate the mechanical properties of adenovirus

Snijder, J., Reddy, V. S., May, E. R., Roos, W. H., Nemerow, G. R. & Wuite, G. J. L., 2013, In : Journal of Virology. 87, 5, p. 2756-2766

Research output: Contribution to JournalArticleAcademicpeer-review

Defensins
Capsid
integrins
Adenoviridae
alpha-Defensins

Probing the biophysical interplay between a viral genome and its capsid

Snijder, J., Uetrecht, C., Rose, R. J., Sanchez-Eugenia, R., Marti, G. A., Agirre, J., Guérin, D. M. A., Wuite, G. J. L., Heck, A. J. R. & Roos, W. H., 2013, In : Nature Chemistry. 5, 6, p. 502-509 8 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Genes
Viruses
Proteins
Mass spectrometry
Life cycle

Role of Mechanical Properties of Cell Mediated Vesicles in Membrane Fusion

Vorselen, D., Roos, W. H., van Loon, J. J. W. A. & Wuite, G. J. L., 2013, In : Biophysical Journal. 104, 2, p. 620A-620A 1 p.

Research output: Contribution to JournalMeeting AbstractOther research output

Open Access

Structural Transitions and Energy Landscape for Cowpea Chlorotic Mottle Virus Capsid Mechanics from Nanomanipulation in Vitro and in Silico

Kononova, O., Snijder, S., Brasch, M., Cornelissen, J., Dima, R. I., Marx, K. A., Wuite, G. J. L., Roos, W. H. & Barsegov, V., 2013, In : Biophysical Journal. 105, 8, p. 1893-1903

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Bromovirus
Capsid
Mechanics
Computer Simulation
Virus Assembly
2012

Mechanics of bacteriophage maturation

Roos, W. H., Gertsman, I., May, E. R., Brooks, C. L., Johnson, J. E. & Wuite, G. J. L., 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 7, p. 2342-2347 6 p.

Research output: Contribution to JournalArticleAcademicpeer-review

bacteriophage
mechanics
maturation
Young modulus
fatigue

Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node

Heuveling, D. A., Visser, G. W. M., de Groot, M., de Boer, J. F., Salumbides - Baclayon, M., Roos, W. H., Wuite, G. J. L., Leemans, C. R., de Bree, R. & van Dongen, G. A. M. S., 2012, In : European Journal of Nuclear Medicine and Molecular Imaging. 39, 7, p. 1161-1168 8 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
Indocyanine Green
Albumins
Serum Albumin
Fluorescence
Injections

Probing the impact of loading rate on the mechanical properties of viral nanoparticles

Snijder, J., Ivanovska, I. L., Salumbides - Baclayon, M., Roos, W. H. & Wuite, G. J. L., 2012, In : Micron. 43, 12, p. 1343-1350 8 p.

Research output: Contribution to JournalArticleAcademicpeer-review

loading rate
Atomic Force Microscopy
Capsid
Viruses
Nanoparticles

Unlocking Internal Prestress from Protein Nanoshells

Klug, W. S., Roos, W. H. & Wuite, G. J. L., 2012, In : Physical Review Letters. 109, 16, 168104.

Research output: Contribution to JournalArticleAcademicpeer-review

Open Access
File
viruses
apexes
elastic properties
proteins
incompatibility
2011

(89)Zr-Nanocolloidal Albumin-Based PET/CT Lymphoscintigraphy for Sentinel Node Detection in Head and Neck Cancer: Preclinical Results

Heuveling, D. A., Visser, G. W. M., Salumbides - Baclayon, M., Roos, W. H., Wuite, G. J. L., Hoekstra, O. S., Leemans, C. R., de Bree, R. & van Dongen, G. A. M. S., 2011, In : Journal of Nuclear Medicine. 52, 10, p. 1580-1584 5 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Lymphoscintigraphy
Head and Neck Neoplasms
Albumins
Neoplasms
cyhalothrin

Effects of Salts on Internal DNA Pressure and Mechanical Properties of Phage Capsids

Evilevitch, A., Roos, W. H., Ivanovska, I. L., Jeembaeva, M., Jonsson, B. & Wuite, G. J. L., 2011, In : Journal of Molecular Biology. 405, 1, p. 18-23 6 p.

Research output: Contribution to JournalArticleAcademicpeer-review

Capsid
Bacteriophages
Salts
Pressure
Atomic Force Microscopy

How to perform a nanoindentation experiment on a virus

Roos, W. H., 2011, Single Molecule Analysis, Series Methods in Molecular Biology. Peterman, EJG. & Wuite, GJL. (eds.). New York: Humama press, 2011 p.

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Prestress strengthens the shell of norwalk virus nanoparticles

Baclayon, M., Shoemaker, G. K., Uetrecht, C., Crawford, S. E., Estes, M. K., Prasad, B. V. V., Heck, A. J. R., Wuite, G. J. L. & Roos, W. H., 2011, In : Nano Letters. 11, 11, p. 4865 9 p.

Research output: Contribution to JournalArticleAcademicpeer-review

viruses
Viruses
Nanoparticles
nanoparticles
Mechanical stability

Voelen en Vervormen van Virussen

Snijder, J., Wuite, G. J. L. & Roos, W. H., 2011, In : Nederlands Tijdschrift voor Natuurkunde. 77, p. 70-74 5 p.

Research output: Contribution to JournalArticleAcademicpeer-review