16S rDNA-based characterization of the BTX-catabolizing microbial associations isolated from a South African sandy oil.

T.K. Ralebitso, W.F.M. Roling, M. Braster, E. Senior, H.W. van Verseveld

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In the presence of different selection pressures, particularly pH and electron donor concentration, indigenous microbial associations which catabolize selected petroleum hydrocarbon components (benzene, toluene and o-, m- and p-xylene (BTX)) were enriched and isolated from a petroleum hydrocarbon-contaminated KwaZulu-Natal sandy soil. Electron microscopy revealed that, numerically, rods constituted the majority of the populations responsible for BTX catabolism. Molecular techniques (polymerase chain reaction (PCR) and 16S rDNA fingerprinting by denaturing-gradient gel electrophoresis (DGGE)) were employed to explore the diversities and analyze the structures of the isolated microbial associations. Pearson product-moment correlation indicated that the different, but chemically similar, petroleum hydrocarbon molecules, effected the isolation of different associations. However, some similar numerically-dominant bands characterized the associations. A 30% similarity was evident between the m- and o-xylene-catabolizing associations regardless of the molecule concentration and the enrichment pH. PCR-DGGE was also used to complement conventional culture-based microbiological procedures for environmental parameter optimization. Band pattern differences indicated profile variations of the isolated associations which possibly accounted for the growth rate changes recorded in response to pH and temperature perturbations.
Original languageEnglish
Pages (from-to)351-357
JournalBiodegradation
Volume11
DOIs
Publication statusPublished - 2001

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Petroleum
petroleum hydrocarbon
Hydrocarbons
Ribosomal DNA
Denaturing Gradient Gel Electrophoresis
Oils
Crude oil
Polymerase chain reaction
xylene
Xylene
Electrophoresis
polymerase chain reaction
oil
electrokinesis
Gels
gel
Polymerase Chain Reaction
Molecules
catabolism
Toluene

Cite this

Ralebitso, T.K. ; Roling, W.F.M. ; Braster, M. ; Senior, E. ; van Verseveld, H.W. / 16S rDNA-based characterization of the BTX-catabolizing microbial associations isolated from a South African sandy oil. In: Biodegradation. 2001 ; Vol. 11. pp. 351-357.
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title = "16S rDNA-based characterization of the BTX-catabolizing microbial associations isolated from a South African sandy oil.",
abstract = "In the presence of different selection pressures, particularly pH and electron donor concentration, indigenous microbial associations which catabolize selected petroleum hydrocarbon components (benzene, toluene and o-, m- and p-xylene (BTX)) were enriched and isolated from a petroleum hydrocarbon-contaminated KwaZulu-Natal sandy soil. Electron microscopy revealed that, numerically, rods constituted the majority of the populations responsible for BTX catabolism. Molecular techniques (polymerase chain reaction (PCR) and 16S rDNA fingerprinting by denaturing-gradient gel electrophoresis (DGGE)) were employed to explore the diversities and analyze the structures of the isolated microbial associations. Pearson product-moment correlation indicated that the different, but chemically similar, petroleum hydrocarbon molecules, effected the isolation of different associations. However, some similar numerically-dominant bands characterized the associations. A 30{\%} similarity was evident between the m- and o-xylene-catabolizing associations regardless of the molecule concentration and the enrichment pH. PCR-DGGE was also used to complement conventional culture-based microbiological procedures for environmental parameter optimization. Band pattern differences indicated profile variations of the isolated associations which possibly accounted for the growth rate changes recorded in response to pH and temperature perturbations.",
author = "T.K. Ralebitso and W.F.M. Roling and M. Braster and E. Senior and {van Verseveld}, H.W.",
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16S rDNA-based characterization of the BTX-catabolizing microbial associations isolated from a South African sandy oil. / Ralebitso, T.K.; Roling, W.F.M.; Braster, M.; Senior, E.; van Verseveld, H.W.

In: Biodegradation, Vol. 11, 2001, p. 351-357.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - 16S rDNA-based characterization of the BTX-catabolizing microbial associations isolated from a South African sandy oil.

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AU - Senior, E.

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