TY - JOUR
T1 - 16S rDNA sequencing and metadata of Dutch dental unit water
AU - Hoogenkamp, Michel A.
AU - Brandt, Bernd W.
AU - Laheij, Alexa M.G.A.
AU - de Soet, Johannes J.
AU - Crielaard, Wim
N1 - Publisher Copyright:
© 2021
PY - 2021/8
Y1 - 2021/8
N2 - Dental practices were approached to fill out a questionnaire on the infection control protocols in use to control biofilm growth in the dental unit and to send two types of water sample. Sampling of the dental units had to be performed prior to any infection control measures and on the second day of operation, to avoid residual effects of biofilm disinfection protocols performed in the weekend. Instructions were given on how to sample the units. Only samples, accompanied with a completed questionnaire and returned within two days by regular mail, were analysed. Samples were processed for heterotrophic plate counts, 16S (V4) rDNA microbiome sequencing and q-PCR for the concentration of bacterial 16S rDNA, fungal 18S rDNA, Legionella spp. and the presence of amoeba. The files contain the metadata needed to interpret and analyse the microbiome data. This dataset can be used by other scientists, members of infection control units, (trainee) bioinformaticians and policy makers. This dataset can provide leads to further unexplored parameters which could influence the microbial ecology of the dental unit.
AB - Dental practices were approached to fill out a questionnaire on the infection control protocols in use to control biofilm growth in the dental unit and to send two types of water sample. Sampling of the dental units had to be performed prior to any infection control measures and on the second day of operation, to avoid residual effects of biofilm disinfection protocols performed in the weekend. Instructions were given on how to sample the units. Only samples, accompanied with a completed questionnaire and returned within two days by regular mail, were analysed. Samples were processed for heterotrophic plate counts, 16S (V4) rDNA microbiome sequencing and q-PCR for the concentration of bacterial 16S rDNA, fungal 18S rDNA, Legionella spp. and the presence of amoeba. The files contain the metadata needed to interpret and analyse the microbiome data. This dataset can be used by other scientists, members of infection control units, (trainee) bioinformaticians and policy makers. This dataset can provide leads to further unexplored parameters which could influence the microbial ecology of the dental unit.
KW - 16S rDNA sequencing
KW - Amoeba
KW - Bacteria
KW - Dental unit water
KW - Fungi
KW - Heterotrophic plate counts
KW - Legionella
UR - http://www.scopus.com/inward/record.url?scp=85107726373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85107726373&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2021.107221
DO - 10.1016/j.dib.2021.107221
M3 - Article
AN - SCOPUS:85107726373
SN - 2352-3409
VL - 37
SP - 1
EP - 5
JO - Data in brief
JF - Data in brief
M1 - 107221
ER -
