16S rDNA sequencing and metadata of Dutch dental unit water

Michel A. Hoogenkamp*, Bernd W. Brandt, Alexa M.G.A. Laheij, Johannes J. de Soet, Wim Crielaard

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review


Dental practices were approached to fill out a questionnaire on the infection control protocols in use to control biofilm growth in the dental unit and to send two types of water sample. Sampling of the dental units had to be performed prior to any infection control measures and on the second day of operation, to avoid residual effects of biofilm disinfection protocols performed in the weekend. Instructions were given on how to sample the units. Only samples, accompanied with a completed questionnaire and returned within two days by regular mail, were analysed. Samples were processed for heterotrophic plate counts, 16S (V4) rDNA microbiome sequencing and q-PCR for the concentration of bacterial 16S rDNA, fungal 18S rDNA, Legionella spp. and the presence of amoeba. The files contain the metadata needed to interpret and analyse the microbiome data. This dataset can be used by other scientists, members of infection control units, (trainee) bioinformaticians and policy makers. This dataset can provide leads to further unexplored parameters which could influence the microbial ecology of the dental unit.

Original languageEnglish
Article number107221
Pages (from-to)1-5
Number of pages5
JournalData in brief
Early online date12 Jun 2021
Publication statusPublished - Aug 2021

Bibliographical note

Publisher Copyright:
© 2021


  • 16S rDNA sequencing
  • Amoeba
  • Bacteria
  • Dental unit water
  • Fungi
  • Heterotrophic plate counts
  • Legionella


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