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• 2010

  Cytochrome P450 BM3 Mutants as NOVEL Biocatalytic TOOLS for Regioselective Hydroxylation of Steroids

  Rea, V., Kolkman, J. A., Vottero, E., Honing, M., Wijmenga, S., Vermeulen, N. P. E. & Commandeur, J. N. M., Aug 2010, In: Drug Metabolism Reviews. 42, p. 285-286

  Research output: Contribution to Journal › Article › Professional

• Determination and identification of estrogenic compounds generated with biosynthetic enzymes using hyphenated screening assays, high resolution mass spectrometry and off-line NMR

  de Vlieger, J. S. B., Kolkman, A. J., Ampt, K. A. M., Commandeur, J. N. M., Vermeulen, N. P. E., Kool, J., Wijmenga, S. S., Niessen, W. M. A., Irth, H. & Honing, M., 2010, In: Journal of Chromatography B. 878, 7-8, p. 667-674 8 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Liquid chromatography
  • Mass spectrometry
  • Nuclear magnetic resonance
  • Assays
  • Liquid Chromatography

• Effect of Organic Solvents on BM3-P450 Biotransformation

  Sun, L., Decker, C., Grootenhuis, P., Saunders, J., Commandeur, J. N. M., Reinen, J. & Vermeulen, N., Aug 2010, In: Drug Metabolism Reviews. 42, p. 83-84

  Research output: Contribution to Journal › Article › Professional

• Role of human glutathione S-transferases in the inactivation of reactive metabolites of clozapine.

  Dragovic, S., Boerma, J. S., van Bergen, L., Vermeulen, N. P. E. & Commandeur, J. N. M., 2010, In: Chemical Research in Toxicology. 23, 9, p. 1467-1476

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Metabolites
  • Clozapine
  • Glutathione Transferase
  • Detoxification
  • Pharmaceutical Preparations

• Role of Human Glutathione S-Transferases on the Inactivation of Reactive Drug METABOLITES of Clozapine

  Dragovic, S., Boerma, J. S., van Bergen, L., Vermeulen, N. P. E. & Commandeur, J. N. M., Aug 2010, In: Drug Metabolism Reviews. 42, p. 67-67

  Research output: Contribution to Journal › Article › Professional
• 2012

  Active Site Substitution A82W Improves the Regioselectivity of Steroid Hydroxylation by Cytochrome P450 BM3 Mutants As Rationalized by Spin Relaxation Nuclear Magnetic Resonance Studies

  Rea, V., Kolkman, A. J., Vottero, E. R., Stronks, E. J., Ampt, K. A., Honing, M., Vermeulen, N. P. E., Wijmenga, S. S. & Commandeur, J. N. M., 2012, In: Biochemistry. 51, 3, p. 750-760

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Regioselectivity
  • Hydroxylation
  • Nuclear magnetic resonance
  • Substitution reactions
  • Cytochrome P-450 Enzyme System

• A single active site mutation inverts stereoselectivity of 16-hydroxylation of testosterone catalyzed by engineered cytochrome P450 BM3.

  Venkataraman, H., de Beer, SB., Bergen, LA., van Essen, N., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In: ChemBioChem. 13, 4, p. 520-523

  Research output: Contribution to Journal › Article › Academic › peer-review

• Mass Spectrometric Characterization of Protein Adducts of Multiple P450-Dependent Reactive Intermediates of Diclofenac to Human Glutathione-S-transferase P1-1

  Boerma, J. S., Dragovic, S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In: Chemical Research in Toxicology. 25, 11, p. 2532-2541

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Glutathione S-Transferase pi
  • Diclofenac
  • Glutathione Transferase
  • hydroxide ion
  • Proteins

• Regio- and Stereoselective Hydroxylation of Optically Active α-Ionone Enantiomers by Engineered Cytochrome P450 BM3 Mutants

  Venkataraman, H., de Beer, S. B. A., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In: Advanced Synthesis and Catalysis. 354, 11-12, p. 2172-2184

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Norisoprenoids
  • Cytochrome P-450 Enzyme System
  • Hydroxylation
  • Enantiomers
  • Secondary Metabolism

• The role of protein plasticity in computational rationalization studies on regioselectivity in testosterone hydroxylation by Cytochrome P450 BM3 mutants

  de Beer, S. B. A., van Bergen, L. A. H., Keijzer, K., Rea, V., Venkataraman, H., Fonseca Guerra, C., Bickelhaupt, F. M., Vermeulen, N. P. E., Commandeur, J. N. M. & Geerke, D. P., 2012, In: Current Drug Metabolism. 13, 2, p. 155-166

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Regioselectivity
  • Hydroxylation
  • Cytochrome P-450 Enzyme System
  • Plasticity
  • Testosterone
• 2014

  Application of engineered cytochrome P450 mutants as biocatalysts for the synthesis of benzylic and aromatic metabolites of fenamic acid NSAIDs

  Venkataraman, H., Verkade-Vreeker, M. C. A., Capoferri, L., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 15 Oct 2014, In: Bioorganic and Medicinal Chemistry. 22, 20, p. 5613-5620

  Research output: Contribution to Journal › Article › Academic › peer-review

• Cytochrome P450-mediated bioactivation of mefenamic acid to quinoneimine intermediates and inactivation by human glutathione S-transferases

  Venkataraman, H., Den Braver, M. W., Vermeulen, N. P. E. & Commandeur, J. N. M., 15 Dec 2014, In: Chemical Research in Toxicology. 27, 12, p. 2071-2081 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Mefenamic Acid
  • Glutathione Transferase
  • Cytochrome P-450 Enzyme System
  • Hydroxy Acids
  • Metabolites

• Effect of human glutathione S-transferase hGSTP1-1 polymorphism on the detoxification of reactive metabolites of clozapine, diclofenac and acetaminophen

  Dragovic, S., Venkataraman, H., Begheijn, S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2014, In: Toxicology Letters. 224, 2, p. 272-281

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Detoxification
  • Metabolites
  • Polymorphism
  • Diclofenac
  • Clozapine

• Human NAD(P)H:quinone oxidoreductase 1 (NQO1)-mediated inactivation of reactive quinoneimine metabolites of diclofenac and mefenamic acid

  Vredenburg, G., Elias, N. S., Venkataraman, H., Hendriks, D. F. G., Vermeulen, N. P. E., Commandeur, J. N. M. & Vos, J. C., 21 Apr 2014, In: Chemical Research in Toxicology. 27, 4, p. 576-86 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • human NQO1 protein
  • Mefenamic Acid
  • Metabolites
  • Diclofenac
  • Acetaminophen

• One-electron oxidation of diclofenac by human cytochrome P450s as a potential bioactivation mechanism for formation of 2'-(glutathion-S-yl)-deschloro-diclofenac.

  Boerma, J. S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2014, In: Chemico-Biological Interactions. 207, p. 32-40

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Diclofenac
  • Cytochromes
  • Glutathione
  • Oxidation
  • Electrons
• 2015

  Activation of the anticancer drugs cyclophosphamide and ifosfamide by cytochrome P450 BM3 mutants

  Vredenburg, G., den Braver-Sewradj, S., van Vugt-Lussenburg, B. M. A., Vermeulen, N. P. E., Commandeur, J. N. M. & Vos, J. C., 5 Jan 2015, In: Toxicology Letters. 232, 1, p. 182-92 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Ifosfamide
  • Metabolic Activation
  • Cytochrome P-450 Enzyme System
  • Cyclophosphamide
  • Chemical activation

• Application of a Continuous-Flow Bioassay to Investigate the Organic Solvent Tolerability of Cytochrome P450 BM3 Mutants

  Reinen, J., van Hemert, D., Vermeulen, N. P. E. & Commandeur, J. N. M., Dec 2015, In: Journal of Biomolecular Screening. 20, 10, p. 1246-55 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Bioassay
  • Organic solvents
  • Mixed Function Oxygenases
  • Biological Assay
  • Cytochrome P-450 Enzyme System

• Biosynthesis of a steroid metabolite by an engineered Rhodococcus erythropolis strain expressing a mutant cytochrome P450 BM3 enzyme

  Venkataraman, H., te Poele, E. M., Rosłoniec, K. Z., Vermeulen, N., Commandeur, J. N. M., van der Geize, R. & Dijkhuizen, L., 18 Jun 2015, In: Applied Microbiology and Biotechnology. 99, 11, p. 4713-4721 9 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Rhodococcus
  • Cytochrome P-450 Enzyme System
  • Steroids
  • Enzymes
  • 17-Ketosteroids

• Characterization of human cytochrome P450s involved in the bioactivation of tri-Ortho-Cresyl phosphate (ToCP)

  Reinen, J., Nematollahi, L., Fidder, A., Vermeulen, N. P. E., Noort, D. & Commandeur, J. N. M., 20 Apr 2015, In: Chemical Research in Toxicology. 28, 4, p. 711-721 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Cytochromes
  • 2-(2-cresyl)-4H-1-3-2-benzodioxaphosphorin-2-oxide
  • Phosphates
  • Butyrylcholinesterase
  • Metabolites

• Linear Interaction Energy Based Prediction of Cytochrome P450 1A2 Binding Affinities with Reliability Estimation

  Capoferri, L., Verkade-Vreeker, M. C. A., Buitenhuis, D., Commandeur, J. N. M., Pastor, M., Vermeulen, N. P. E. & Geerke, D. P., 9 Nov 2015, In: PLoS ONE. 10, 11

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

• Mini-dialysis tubes as tools to prepare drug-protein adducts of P450-dependent reactive drug metabolites

  Boerma, J. S., Elias, N. S., Vermeulen, N. P. E. & Commandeur, J. N. M., 25 Jan 2015, In: Journal of Pharmaceutical and Biomedical Analysis. 103, p. 17-25

  Research output: Contribution to Journal › Article › Academic › peer-review

• Selective whole-cell biosynthesis of the designer drug metabolites 15- or 16-betahydroxynorethisterone by engineered Cytochrome P450 BM3 mutants

  Reinen, J., Vredenburg, G., Klaering, K., Vermeulen, N. P. E., Commandeur, J. N. M., Honing, M. & Vos, J. C., 1 Nov 2015, In: Journal of Molecular Catalysis B. Enzymatic. 121, p. 64-74 11 p., 3212.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Cytochrome P-450 Enzyme System
  • Hydroxylation
  • Biosynthesis
  • Designer Drugs
  • Norethindrone
• 2019

  Characterization of kinetics of human cytochrome P450s involved in bioactivation of flucloxacillin: inhibition of CYP3A-catalysed hydroxylation by sulfaphenazole

  Dekker, S. J., Dohmen, F., Vermeulen, N. P. E. & Commandeur, J. N. M., 1 Feb 2019, In: British Journal of Pharmacology. 176, 3, p. 466-477 12 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Sulfaphenazole
  • Floxacillin
  • Hydroxylation
  • Cytochrome P-450 CYP3A
  • Cytochromes

• Evaluation of Luminogenic Substrates as Probe Substrates for Bacterial Cytochrome P450 Enzymes: Application to Mycobacterium tuberculosis

  Ortega Ugalde, S., Ma, D., Cali, J. J. & Commandeur, J. N. M., 1 Aug 2019, In: SLAS Discovery. 24, 7, p. 745-754 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Mycobacterium tuberculosis
  • Cytochrome P-450 Enzyme System
  • Substrates
  • Luminescence
  • Lanosterol

• Function, essentiality, and expression of cytochrome P450 enzymes and their cognate redox partners in Mycobacterium tuberculosis: are they drug targets?

  Ortega Ugalde, S., Boot, M., Commandeur, J. N. M., Jennings, P., Bitter, W. & Vos, J. C., 2 May 2019, In: Applied Microbiology and Biotechnology. 103, 9, p. 3597-3614 18 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Mycobacterium tuberculosis
  • Cytochrome P-450 Enzyme System
  • Oxidation-Reduction
  • Pharmaceutical Preparations
  • Ferredoxins

• Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol

  Frydenvang, K., Verkade-Vreeker, M. C. A., Dohmen, F., Commandeur, J. N. M., Rafiq, M., Mirza, O., Jørgensen, F. S. & Geerke, D. P., 1 May 2019, In: PLoS ONE. 14, 5, e0217292.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Dithiothreitol
  • dithiothreitol
  • Structural analysis
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450