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• The role of protein plasticity in computational rationalization studies on regioselectivity in testosterone hydroxylation by Cytochrome P450 BM3 mutants

  de Beer, S. B. A., van Bergen, L. A. H., Keijzer, K., Rea, V., Venkataraman, H., Fonseca Guerra, C., Bickelhaupt, F. M., Vermeulen, N. P. E., Commandeur, J. N. M. & Geerke, D. P., 2012, In: Current Drug Metabolism. 13, 2, p. 155-166

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Regioselectivity
  • Hydroxylation
  • Cytochrome P-450 Enzyme System
  • Plasticity
  • Testosterone

• Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol

  Frydenvang, K., Verkade-Vreeker, M. C. A., Dohmen, F., Commandeur, J. N. M., Rafiq, M., Mirza, O., Jørgensen, F. S. & Geerke, D. P., 1 May 2019, In: PLoS ONE. 14, 5, e0217292.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Dithiothreitol
  • dithiothreitol
  • Structural analysis
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450

• Selective whole-cell biosynthesis of the designer drug metabolites 15- or 16-betahydroxynorethisterone by engineered Cytochrome P450 BM3 mutants

  Reinen, J., Vredenburg, G., Klaering, K., Vermeulen, N. P. E., Commandeur, J. N. M., Honing, M. & Vos, J. C., 1 Nov 2015, In: Journal of Molecular Catalysis B. Enzymatic. 121, p. 64-74 11 p., 3212.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Cytochrome P-450 Enzyme System
  • Hydroxylation
  • Biosynthesis
  • Designer Drugs
  • Norethindrone

• Role of Human Glutathione S-Transferases on the Inactivation of Reactive Drug METABOLITES of Clozapine

  Dragovic, S., Boerma, J. S., van Bergen, L., Vermeulen, N. P. E. & Commandeur, J. N. M., Aug 2010, In: Drug Metabolism Reviews. 42, p. 67-67

  Research output: Contribution to Journal › Article › Professional

• Role of human glutathione S-transferases in the inactivation of reactive metabolites of clozapine.

  Dragovic, S., Boerma, J. S., van Bergen, L., Vermeulen, N. P. E. & Commandeur, J. N. M., 2010, In: Chemical Research in Toxicology. 23, 9, p. 1467-1476

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Metabolites
  • Clozapine
  • Glutathione Transferase
  • Detoxification
  • Pharmaceutical Preparations

• Regio- and Stereoselective Hydroxylation of Optically Active α-Ionone Enantiomers by Engineered Cytochrome P450 BM3 Mutants

  Venkataraman, H., de Beer, S. B. A., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In: Advanced Synthesis and Catalysis. 354, 11-12, p. 2172-2184

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Norisoprenoids
  • Cytochrome P-450 Enzyme System
  • Hydroxylation
  • Enantiomers
  • Secondary Metabolism

• Mini-dialysis tubes as tools to prepare drug-protein adducts of P450-dependent reactive drug metabolites

  Boerma, J. S., Elias, N. S., Vermeulen, N. P. E. & Commandeur, J. N. M., 25 Jan 2015, In: Journal of Pharmaceutical and Biomedical Analysis. 103, p. 17-25

  Research output: Contribution to Journal › Article › Academic › peer-review

• Mass Spectrometric Characterization of Protein Adducts of Multiple P450-Dependent Reactive Intermediates of Diclofenac to Human Glutathione-S-transferase P1-1

  Boerma, J. S., Dragovic, S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In: Chemical Research in Toxicology. 25, 11, p. 2532-2541

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Glutathione S-Transferase pi
  • Diclofenac
  • Glutathione Transferase
  • hydroxide ion
  • Proteins

• Linear Interaction Energy Based Prediction of Cytochrome P450 1A2 Binding Affinities with Reliability Estimation

  Capoferri, L., Verkade-Vreeker, M. C. A., Buitenhuis, D., Commandeur, J. N. M., Pastor, M., Vermeulen, N. P. E. & Geerke, D. P., 9 Nov 2015, In: PLoS ONE. 10, 11

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

• Function, essentiality, and expression of cytochrome P450 enzymes and their cognate redox partners in Mycobacterium tuberculosis: are they drug targets?

  Ortega Ugalde, S., Boot, M., Commandeur, J. N. M., Jennings, P., Bitter, W. & Vos, J. C., 2 May 2019, In: Applied Microbiology and Biotechnology. 103, 9, p. 3597-3614 18 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Mycobacterium tuberculosis
  • Cytochrome P-450 Enzyme System
  • Oxidation-Reduction
  • Pharmaceutical Preparations
  • Ferredoxins

• Evaluation of Luminogenic Substrates as Probe Substrates for Bacterial Cytochrome P450 Enzymes: Application to Mycobacterium tuberculosis

  Ortega Ugalde, S., Ma, D., Cali, J. J. & Commandeur, J. N. M., 1 Aug 2019, In: SLAS Discovery. 24, 7, p. 745-754 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Mycobacterium tuberculosis
  • Cytochrome P-450 Enzyme System
  • Substrates
  • Luminescence
  • Lanosterol

• Effect of Organic Solvents on BM3-P450 Biotransformation

  Sun, L., Decker, C., Grootenhuis, P., Saunders, J., Commandeur, J. N. M., Reinen, J. & Vermeulen, N., Aug 2010, In: Drug Metabolism Reviews. 42, p. 83-84

  Research output: Contribution to Journal › Article › Professional

• Determination and identification of estrogenic compounds generated with biosynthetic enzymes using hyphenated screening assays, high resolution mass spectrometry and off-line NMR

  de Vlieger, J. S. B., Kolkman, A. J., Ampt, K. A. M., Commandeur, J. N. M., Vermeulen, N. P. E., Kool, J., Wijmenga, S. S., Niessen, W. M. A., Irth, H. & Honing, M., 2010, In: Journal of Chromatography B. 878, 7-8, p. 667-674 8 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Liquid chromatography
  • Mass spectrometry
  • Nuclear magnetic resonance
  • Assays
  • Liquid Chromatography

• Cytochrome P450 BM3 Mutants as NOVEL Biocatalytic TOOLS for Regioselective Hydroxylation of Steroids

  Rea, V., Kolkman, J. A., Vottero, E., Honing, M., Wijmenga, S., Vermeulen, N. P. E. & Commandeur, J. N. M., Aug 2010, In: Drug Metabolism Reviews. 42, p. 285-286

  Research output: Contribution to Journal › Article › Professional

• Characterization of kinetics of human cytochrome P450s involved in bioactivation of flucloxacillin: inhibition of CYP3A-catalysed hydroxylation by sulfaphenazole

  Dekker, S. J., Dohmen, F., Vermeulen, N. P. E. & Commandeur, J. N. M., 1 Feb 2019, In: British Journal of Pharmacology. 176, 3, p. 466-477 12 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Sulfaphenazole
  • Floxacillin
  • Hydroxylation
  • Cytochrome P-450 CYP3A
  • Cytochromes

• Characterization of human cytochrome P450s involved in the bioactivation of tri-Ortho-Cresyl phosphate (ToCP)

  Reinen, J., Nematollahi, L., Fidder, A., Vermeulen, N. P. E., Noort, D. & Commandeur, J. N. M., 20 Apr 2015, In: Chemical Research in Toxicology. 28, 4, p. 711-721 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Cytochromes
  • 2-(2-cresyl)-4H-1-3-2-benzodioxaphosphorin-2-oxide
  • Phosphates
  • Butyrylcholinesterase
  • Metabolites

• Biosynthesis of a steroid metabolite by an engineered Rhodococcus erythropolis strain expressing a mutant cytochrome P450 BM3 enzyme

  Venkataraman, H., te Poele, E. M., Rosłoniec, K. Z., Vermeulen, N., Commandeur, J. N. M., van der Geize, R. & Dijkhuizen, L., 18 Jun 2015, In: Applied Microbiology and Biotechnology. 99, 11, p. 4713-4721 9 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Rhodococcus
  • Cytochrome P-450 Enzyme System
  • Steroids
  • Enzymes
  • 17-Ketosteroids

• A single active site mutation inverts stereoselectivity of 16-hydroxylation of testosterone catalyzed by engineered cytochrome P450 BM3.

  Venkataraman, H., de Beer, SB., Bergen, LA., van Essen, N., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In: ChemBioChem. 13, 4, p. 520-523

  Research output: Contribution to Journal › Article › Academic › peer-review

• Application of a Continuous-Flow Bioassay to Investigate the Organic Solvent Tolerability of Cytochrome P450 BM3 Mutants

  Reinen, J., van Hemert, D., Vermeulen, N. P. E. & Commandeur, J. N. M., Dec 2015, In: Journal of Biomolecular Screening. 20, 10, p. 1246-55 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Bioassay
  • Organic solvents
  • Mixed Function Oxygenases
  • Biological Assay
  • Cytochrome P-450 Enzyme System

• Active Site Substitution A82W Improves the Regioselectivity of Steroid Hydroxylation by Cytochrome P450 BM3 Mutants As Rationalized by Spin Relaxation Nuclear Magnetic Resonance Studies

  Rea, V., Kolkman, A. J., Vottero, E. R., Stronks, E. J., Ampt, K. A., Honing, M., Vermeulen, N. P. E., Wijmenga, S. S. & Commandeur, J. N. M., 2012, In: Biochemistry. 51, 3, p. 750-760

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Regioselectivity
  • Hydroxylation
  • Nuclear magnetic resonance
  • Substitution reactions
  • Cytochrome P-450 Enzyme System

• Activation of the anticancer drugs cyclophosphamide and ifosfamide by cytochrome P450 BM3 mutants

  Vredenburg, G., den Braver-Sewradj, S., van Vugt-Lussenburg, B. M. A., Vermeulen, N. P. E., Commandeur, J. N. M. & Vos, J. C., 5 Jan 2015, In: Toxicology Letters. 232, 1, p. 182-92 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Ifosfamide
  • Metabolic Activation
  • Cytochrome P-450 Enzyme System
  • Cyclophosphamide
  • Chemical activation