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• Active site substitution A82W improves regioselectivity of steroid hydroxylation by cytochrome P450 BM3 mutants as rationalised by spin relaxation NMR studies

  Rea, V., Kolkman, ER., Vottero, E. R., Stronks, E. J., Ampt, K. A., Honing, M., Vermeulen, N. P. E., Wijmenga, S. S. & Commandeur, J. N. M., 2011, (Accepted/In press) In : The Biochemist.

  Research output: Contribution to Journal › Article › Academic › peer-review

• Active Site Substitution A82W Improves the Regioselectivity of Steroid Hydroxylation by Cytochrome P450 BM3 Mutants As Rationalized by Spin Relaxation Nuclear Magnetic Resonance Studies

  Rea, V., Kolkman, A. J., Vottero, E. R., Stronks, E. J., Ampt, K. A., Honing, M., Vermeulen, N. P. E., Wijmenga, S. S. & Commandeur, J. N. M., 2012, In : Biochemistry. 51, 3, p. 750-760

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Regioselectivity
  • Hydroxylation
  • Nuclear magnetic resonance
  • Substitution reactions
  • Cytochrome P-450 Enzyme System

• AMAP, the alleged non-toxic isomer of acetaminophen, is toxic in rat and human liver

  Hadi, M., Dragovic, S., van Swelm, R., Herpers, B., van de Water, B., Russel, RG., Commandeur, J. N. M. & Groothuis, G. M., 2013, In : Archives of Toxicology. 87, 1, p. 155-165

  Research output: Contribution to Journal › Article › Academic › peer-review

  • 3-aminophenol
  • Isomers
  • Liver
  • Rats
  • Acetaminophen

• Application of a cocktail approach to screen cytochrome P450 BM3 libraries for metabolic activity and diversity.

  Reinen, J. R., Postma, G., Tump, C., Bloemberg, T., Engel, J., Vermeulen, N. P. E., Commandeur, J. N. M. & Honing, M., 2016, In : Analytical and Bioanalytical Chemistry. 408, 5, p. 1425-1443 19 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

• Application of a fluorescence-based continuous-flow bioassay to screen for diversity of cytochrome P450 BM3 mutant libraries

  Reinen, J., Ferman, S., Vottero, E. R., Vermeulen, N. & Commandeur, J. N. M., 2011, In : Journal of Biomolecular Screening. 16, 2, p. 239-250 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Bioassay
  • Fluorescence
  • Flow Injection Analysis
  • Biological Assay
  • Cytochrome P-450 Enzyme System

• Application of CYP102A1M11H as a tool for the generation of protein adducts of reactive drug metabolites

  Boerma, J. S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2011, In : Chemical Research in Toxicology. 24, 8, p. 1263-1274 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Metabolites
  • Glutathione S-Transferase pi
  • Cysteine
  • Clozapine
  • Acetaminophen

• Application of cytochrome P450 BM3 mutants as biocatalysts for the profiling of estrogen receptor binding metabolites of the mycotoxin zearalenone

  Reinen, J., Kalma, L., Begheijn, S., Heus, F. A. H., Commandeur, J. N. M. & Vermeulen, N. P. E., 2011, In : Xenobiotica. 41, 1, p. 59-70 11 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Zearalenone
  • Metabolites
  • Mycotoxins
  • Cytochrome P-450 Enzyme System
  • Estrogen Receptors

• A single active site mutation inverts stereoselectivity of 16-hydroxylation of testosterone catalyzed by engineered cytochrome P450 BM3.

  Venkataraman, H., de Beer, SB., Bergen, LA., van Essen, N., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In : ChemBioChem. 13, 4, p. 520-523

  Research output: Contribution to Journal › Article › Academic › peer-review

• Characterisation of Human Cytochrome P450s Involved in the Bioactivation of Clozapine

  Dragovic, S., Gunness, P., Ingelman-Sundberg, M., Vermeulen, N. P. E. & Commandeur, J. N. M., 2013, In : Drug Metabolism and Disposition. 41, 3, p. 651-658

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Clozapine
  • Cytochromes
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 CYP2D6
  • Liver

• Characterization of cytochrome P450 isoforms involved in sequential two-step bioactivation of diclofenac to reactive p-benzoquinone imines.

  den Braver, M. W., Sewradj, S. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2016, In : Toxicology Letters. 253, p. 46-54

  Research output: Contribution to Journal › Article › Academic › peer-review

• Characterization of human cytochrome P450 mediated bioactivation of amodiaquine and its major metabolite N-desethylamodiaquine

  Zhang, Y., Vermeulen, N. P. E. & Commandeur, J. N. M., 1 Mar 2017, In : British Journal of Clinical Pharmacology. 83, 3, p. 572-583 12 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • desethylamodiaquine
  • Amodiaquine
  • Cytochrome P-450 Enzyme System
  • Liver Microsomes
  • Cytochromes

• Combination of biotransformation by P450 BM3 mutants with on-line post-column bioaffinity and mass spectrometric profiling as a novel strategy to diversify and characterize p38α kinase inhibitors.

  Rea, V., Falck, D., Kool, J., de Kanter, F. J. J., Commandeur, J. N. M., Vermeulen, N. P. E., Niessen, W. M. A. & Honing, M., 2013, In : MedChemComm. 4, p. 371-377

  Research output: Contribution to Journal › Article › Academic › peer-review

  • N-(4-(2-ethyl-4-(3-methylphenyl)-1,3-thiazol-5-yl)-2-pyridyl)benzamide
  • Biotransformation
  • p38 Mitogen-Activated Protein Kinases
  • Screening
  • Lead compounds

• Diclofenac inhibits tumor necrosis factor-a-induced nuclear factor-kB activation causing synergic hepatocyte apoptosis

  Frederiksson, L., Herpers, B., Benedetti, G., Matadin, Q., Puigvert, J. C., de Bont, H., Dragovic, S., Vermeulen, N. P. E., Commandeur, J. N. M., Danen, E., de Graauw, M. & van de Water, B., 2011, In : Hepatology. 53, 6, p. 383-394 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Diclofenac
  • Hepatocytes
  • Tumor Necrosis Factor-alpha
  • Apoptosis
  • NF-kappa B

• Different reacive metabolites of nevirapine require distinct glutathione S-tranferase isoforms for bioinactivation.

  Dekker, S. J., Zhang, Y., Vos, J. C., Vermeulen, N. P. E. & Commandeur, J. N. M., 2016, In : Chemical Research in Toxicology. 29, 12, p. 2136-2144

  Research output: Contribution to Journal › Article › Academic › peer-review

• Different reactive metabolites of nevirapine require distinct glutathione S-transferase isoforms for bio-inactivation

  Dekker, S. J., Vos, J., Vermeulen, N. P. & Commandeur, J. N., 16 Sep 2016, In : Toxicology Letters. 29, 12, p. 2136-2144 9 p.

  Research output: Contribution to Journal › Article › Professional

• Direct comparison of UDP-glucuronosyltransferase and cytochrome P450 activities in human liver microsomes, plated and suspended primary human hepatocytes from five liver donors

  den Braver-Sewradj, S. P., den Braver, M. W., Baze, A., Decorde, J., Fonsi, M., Bachellier, P., Vermeulen, N. P. E., Commandeur, J. N. M., Richert, L. & Vos, J. C., 1 Aug 2017, In : European Journal of Pharmaceutical Sciences. 109, p. 96-110 15 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Glucuronosyltransferase
  • Liver Microsomes
  • Human Activities
  • Cytochrome P-450 Enzyme System
  • Cytochromes

• Effect of human glutathione S-transferases on glutathione-dependent inactivation of cytochrome P450-dependent reactive intermediates of diclofenac.

  Dragovic, S., Boerma, J. S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2013, In : Chemical Research in Toxicology. 26, 11, p. 1632-1641

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Diclofenac
  • Glutathione Transferase
  • Cytochrome P-450 Enzyme System
  • hydroxide ion
  • Glutathione

• Efficient screening of cytochrome P450 BM3 mutants for their metabolic activity and diversity toward a wide set of drug-like molecules in chemical space

  Reinen, J., van Leeuwen, J. S., Li, Y., Sun, L., Grootenhuis, P. D. J., Decker, C. J., Saunders, J., Vermeulen, N. P. E. & Commandeur, J. N. M., 2011, (Accepted/In press) In : Drug Metabolism and Disposition.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Cytochrome P-450 Enzyme System
  • Pharmaceutical Preparations
  • Liquid Chromatography
  • Mass Spectrometry
  • Libraries

• Engineering a self-sufficient Mycobacterium tuberculosis CYP130 by gene fusion with the reductase-domain of CYP102A1 from Bacillus megaterium

  Ortega Ugalde, S., Luirink, R. A., Geerke, D. P., Vermeulen, N. P. E., Bitter, W. & Commandeur, J. N. M., 7 Dec 2017, In : Journal of Inorganic Biochemistry. 180, p. 48 53 p., 180.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • flavocytochrome P450 BM3 monoxygenases
  • Dextromethorphan
  • Demethylation
  • Gene Fusion
  • Mycobacterium tuberculosis

• Generic method for the absolute quantification of glutathione S-conjugates: Application to the conjugates of acetaminophen, clozapine and diclofenac

  den Braver, M. W., Vermeulen, N. P. E. & Commandeur, J. N. M., 1 Mar 2017, In : Journal of Chromatography B. 1046, p. 185-194 10 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • o-Phthalaldehyde
  • Diclofenac
  • Clozapine
  • Acetylcysteine
  • Acetaminophen

• Genetic risk factors for clozapine-induced neutropenia and agranulocytosis in a Dutch psychiatric population.

  Van der Weide, K., Loovers, H., Pondman, K., Bogers, J., van der Straaten, T., Langemeijer, E. V., Cohen, D., Commandeur, J. N. M. & van der Weide, J., 2016, In : Pharmacogenomics Journal.

  Research output: Contribution to Journal › Article › Academic › peer-review

• Human glutathione S-transferases- and NAD(P)H:quinone oxidoreductase 1-catalyzed inactivation of reactive quinoneimines of amodiaquine and N-desethylamodiaquine: Possible implications for susceptibility to amodiaquine-induced liver toxicity

  Zhang, Y., den Braver-Sewradj, S. P., Vos, J. C., Vermeulen, N. P. E. & Commandeur, J. N. M., 2017, In : Toxicology Letters. 275, p. 83-91 9 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • amodiaquine quinoneimine
  • desethylamodiaquine
  • Amodiaquine
  • quinone
  • Liver

• Identification of CYP1A2 ligands by structure-based and ligand-based virtual screening

  Vasanthanathan, P., Lastdrager, J., Oostenbrink, C., Commandeur, J. N. M., Vermeulen, N. P. E., Jorgensen, F. S. & Olsen, L., Sep 2011, In : MedChemComm. 2, 9, p. 853-859

  Research output: Contribution to Journal › Article › Academic › peer-review

• Insights into regioselective metabolism of mefenamic acid by cytochrome P450 BM3 mutants through crystallography, docking, molecular dynamics, and free energy calculations.

  Capoferri, L., Leth, R., ter Haar, E., Mohanty, A. K., Grootenhuis, P. D. J., Vottero, E. R., Commandeur, J. N. M., Vermeulen, N. P. E., Jorgensen, F. S., Olsen, L. & Geerke, D. P., 2016, In : Proteins. 84, 3, p. 383-396

  Research output: Contribution to Journal › Article › Academic › peer-review

• Inter-donor variability of phase I/phase II metabolism of three reference drugs in cryopreserved primary human hepatocytes in suspension and monolayer.

  Sewradj, S. P., den Braver, M. W., Vermeulen, N. P. E., Commandeur, J. N. M., Richert, L. & Vos, J. C., 2016, In : Toxicology in Vitro. 33, p. 71-79 9 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

• Mass Spectrometric Characterization of Protein Adducts of Multiple P450-Dependent Reactive Intermediates of Diclofenac to Human Glutathione-S-transferase P1-1

  Boerma, J. S., Dragovic, S., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In : Chemical Research in Toxicology. 25, 11, p. 2532-2541

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Glutathione S-Transferase pi
  • Diclofenac
  • Glutathione Transferase
  • hydroxide ion
  • Proteins

• Reconstitution of the interplay between cytochrome P450 and human glutathione S-transferases in clozapine metabolism in yeast.

  Vredenburg, G., Vassell, K. P., Commandeur, J. N. M., Vermeulen, N. P. E. & Vos, J. C., 2013, In : Toxicology Letters. 222, 3, p. 247-256

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Metabolism
  • Yeast
  • Clozapine
  • Glutathione Transferase
  • Cytochrome P-450 Enzyme System

• Regio- and Stereoselective Hydroxylation of Optically Active α-Ionone Enantiomers by Engineered Cytochrome P450 BM3 Mutants

  Venkataraman, H., de Beer, S. B. A., Geerke, D. P., Vermeulen, N. P. E. & Commandeur, J. N. M., 2012, In : Advanced Synthesis and Catalysis. 354, 11-12, p. 2172-2184

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Norisoprenoids
  • Cytochrome P-450 Enzyme System
  • Hydroxylation
  • Enantiomers
  • Secondary Metabolism

• Role of residue 87 in substrate selectivity and regioselectivity of drug-metabolizing cytochrome P450 CYP102A1 M11

  Vottero, E. R., Rea, V., Lastdrager, J., Honing, M., Vermeulen, N. P. E. & Commandeur, J. N. M., 2011, In : Journal of Biological Inorganic Chemistry - JBIC. 16, 6, p. 899-912 13 p.

  Research output: Contribution to Journal › Article › Academic › peer-review

  Open Access

  • Regioselectivity
  • flavocytochrome P450 BM3 monoxygenases
  • Cytochrome P-450 Enzyme System
  • Drug Residues
  • Substrates

• Role of residue 87 in the activity and regioselectivity of clozapine metabolism by drug-metabolizing CYP102A1 M11H: application for structural characterization of clozapine GSH conjugates

  Rea, V., Dragovic, S., Boerma, J. S., de Kanter, F. J. J., Vermeulen, N. P. E. & Commandeur, J. N. M., 2011, (Accepted/In press) In : Drug Metabolism and Disposition. p. 39(12): 2411-20. doi: 10.1124/dmd.111.041046.

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Clozapine
  • Pharmaceutical Preparations
  • Amino Acids
  • Demethylation
  • Glutathione Transferase

• Simulation of interindividual differences in inactivation of reactive para-benzoquinone imine metabolites of diclofenac by glutathione S-transferases in human liver cytosol.

  den Braver, M. W., Zhang, Y., Venkataraman, H., Vermeulen, N. P. E. & Commandeur, J. N. M., 2016, In : Toxicology Letters. 255, p. 52-62

  Research output: Contribution to Journal › Article › Academic › peer-review

• The role of protein plasticity in computational rationalization studies on regioselectivity in testosterone hydroxylation by Cytochrome P450 BM3 mutants

  de Beer, S. B. A., van Bergen, L. A. H., Keijzer, K., Rea, V., Venkataraman, H., Fonseca Guerra, C., Bickelhaupt, F. M., Vermeulen, N. P. E., Commandeur, J. N. M. & Geerke, D. P., 2012, In : Current Drug Metabolism. 13, 2, p. 155-166

  Research output: Contribution to Journal › Article › Academic › peer-review

  • Regioselectivity
  • Hydroxylation
  • Cytochrome P-450 Enzyme System
  • Plasticity
  • Testosterone