A brief introduction to single-molecule fluorescence methods

Siet M.J.L. van den Wildenberg, Bram Prevo, Erwin J.G. Peterman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

One of the more popular single-molecule approaches in biological science is single-molecule fluorescence microscopy, which will be the subject of the following section of this volume. Fluorescence methods provide the sensitivity required to study biology on the single-molecule level, but they also allow access to useful measurable parameters on time and length scales relevant for the biomolecular world. Before several detailed experimental approaches will be addressed, we will first give a general overview of single-molecule fluorescence microscopy. We start with discussing the phenomenon of fluorescence in general and the history of single-molecule fluorescence microscopy. Next, we will review fluorescent probes in more detail and the equipment required to visualize them on the single-molecule level. We will end with a description of parameters measurable with such approaches, ranging from protein counting and tracking, single-molecule localization super-resolution microscopy, to distance measurements with Förster Resonance Energy Transfer and orientation measurements with fluorescence polarization.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages93-113
Number of pages21
Volume1665
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1665
ISSN (Print)1064-3745

Cite this

van den Wildenberg, S. M. J. L., Prevo, B., & Peterman, E. J. G. (2018). A brief introduction to single-molecule fluorescence methods. In Methods in Molecular Biology (Vol. 1665, pp. 93-113). (Methods in Molecular Biology; Vol. 1665). Humana Press Inc. DOI: 10.1007/978-1-4939-7271-5_5
van den Wildenberg, Siet M.J.L. ; Prevo, Bram ; Peterman, Erwin J.G./ A brief introduction to single-molecule fluorescence methods. Methods in Molecular Biology. Vol. 1665 Humana Press Inc, 2018. pp. 93-113 (Methods in Molecular Biology).
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van den Wildenberg, SMJL, Prevo, B & Peterman, EJG 2018, A brief introduction to single-molecule fluorescence methods. in Methods in Molecular Biology. vol. 1665, Methods in Molecular Biology, vol. 1665, Humana Press Inc, pp. 93-113. DOI: 10.1007/978-1-4939-7271-5_5

A brief introduction to single-molecule fluorescence methods. / van den Wildenberg, Siet M.J.L.; Prevo, Bram; Peterman, Erwin J.G.

Methods in Molecular Biology. Vol. 1665 Humana Press Inc, 2018. p. 93-113 (Methods in Molecular Biology; Vol. 1665).

Research output: Chapter in Book/Report/Conference proceedingChapter

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van den Wildenberg SMJL, Prevo B, Peterman EJG. A brief introduction to single-molecule fluorescence methods. In Methods in Molecular Biology. Vol. 1665. Humana Press Inc. 2018. p. 93-113. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-7271-5_5