A combined cellomics and proteomics approach to uncover neuronal pathways to psychiatric disorder

Martina Rosato, Titia Gebuis, Iryna Paliukhovich, Sven Stringer, Patrick F. Sullivan, August B. Smit, Ronald E. van Kesteren*

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Studying biological mechanisms underlying neuropsychiatric disorders is highly challenging as many risk genes are associated with these disorders. This complexity requires research approaches to reliably dissect the cell biology of the risk genes involved. Here, we describe a combined cellomics–proteomics approach that allows (a) medium-throughput functional screening and unbiased selection of important risk genes, and (b) discovery of common functional pathways and interactome connections of selected risk genes. The overlay of pathway and proteome data from selected genes in a biological context can be used to formulate new testable hypothesis of both the genetics and the biology of the disorders.

Original languageEnglish
Title of host publicationNeuroproteomics
EditorsKa Wan Li
PublisherHumana Press Inc.
Pages199-215
Number of pages17
Edition2nd
ISBN (Electronic)9781493996629
ISBN (Print)9781493996612
DOIs
Publication statusPublished - 2019

Publication series

NameNeuromethods
Volume146
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Funding

MR was supported by the European grant U-FP7 MC-ITN IN-SENS (#607616) and the Schizophrenia United Network (SUN) project. We gratefully acknowledge support from the Swedish Research Council (Vetenskapsr?det, award D0886501).

FundersFunder number
Schizophrenia United Network
VetenskapsrådetD0886501

    Keywords

    • Cellomics
    • High-content screening
    • Proteomics
    • Psychiatric disorders

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