A cytochrome b5 is required for cytochrome P450 activity and blue flower colours

N. de Vetten, J. ter Horst, H.-P. van Schaik, A.H. de Boer, J.N.M. Mol, R.E. Koes

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    The substitution pattern of anthocyanin pigments is a main determinant of flower color. Flavonoid 3',5'-hydroxylase (F3'5'H) is a cytochrome P450 enzyme (Cyt P450) that catalyzes the 3',5'-hydroxylation of dihydroflavonols, the precursors of purple anthocyanins. Species such as rose and carnation lack F3'5'H activity and are, therefore, unable to generate purple or blue flowers. Petunia, on the other hand, contains two loci, termed hf1 and hf2, that encode a Cyt P450 with F3'5'H activity. Here we report the identification of an additional petunia gene that is required for 3',5' substitution of anthocyanins and purple flower colors. It encodes a cytochrome b
    Original languageEnglish
    Pages (from-to)778-783
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume2
    DOIs
    Publication statusPublished - 1998

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