A Distributed Parameter Approach to Model the Transcriptional Response of Escherichia Coli in a Scale-Down Reactor

Julia Lischke, Kevin Schmidt, Joachim Von Wulffen, Oliver Sawodny

Research output: Chapter in Book / Report / Conference proceedingConference contributionAcademicpeer-review

Abstract

Scale-up is a major challenge in bioprocess engineering. Because physically achievable mixing times in large-scale bioreactors are limited, gradients of dissolved oxygen are likely to occur. Since the impact on performance of production strains in this environment is poorly understood, we address this question by developing a distributed parameter model. This approach makes it possible to model the adaptive behavior of Escherichia coli during reoccurring switches between oxygen free and oxygen rich environments. We use a set of first-order hyperbolic partial differential equations actuated by spatially varying activation dynamics. The design of the distributed parameter model is based on a scale-down bioreactor, which is used for an experimental validation of the simulation results.

Original languageEnglish
Title of host publication2018 IEEE Conference on Control Technology and Applications, CCTA 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages559-564
Number of pages6
ISBN (Electronic)9781538676981
DOIs
Publication statusPublished - 26 Oct 2018
Externally publishedYes
Event2nd IEEE Conference on Control Technology and Applications, CCTA 2018 - Copenhagen, Denmark
Duration: 21 Aug 201824 Aug 2018

Publication series

Name2018 IEEE Conference on Control Technology and Applications, CCTA 2018

Conference

Conference2nd IEEE Conference on Control Technology and Applications, CCTA 2018
CountryDenmark
CityCopenhagen
Period21/08/1824/08/18

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