A dual functional bone-defect-filling material with sequential antibacterial and osteoinductive properties for infected bone defect repair

D. Wang, Y. Liu, Y. Liu, L. Yan, S.A.J. Zaat, D. Wismeijer, J.L. Pathak, G. Wu

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Infected bone defect healing is hindered by infection and compromised bone regenerative capacity. In this study, we designed a dual functional bone‐defect‐filling material with a sequential release system, that is, a burst release of a potent antibacterial agent, hydroxypropyltrimethyl ammonium chloride chitosan (HACC), followed by a controlled release of osteoinductive bone morphogenic protein (BMP2) to repair the infected bone defect. Minimum bactericidal concentration (MBC) of HACC against methicillin‐resistant Staphylococcus aureus was 40 μg/mL. HACC at 40 μg/mL did not affect preosteoblast proliferation and did not influence the BMP2‐induced alkaline phosphatase activity, osteocalcin expression, and matrix mineralization. in vitro release profile revealed burst release of HACC followed by a slow release of BMP2. in vivo bone formation was observed only in the BMP2‐containing groups. HACC did not influence of biomimetic calcium phosphate (BioCaP) resorption and BMP2‐induced bone formation. In conclusion, the optimized HACC/BMP2‐incorporated BioCaP complex showed strong antibacterial effect and robustly enhanced osteoinduction both in vitro and in vivo.
Original languageEnglish
Pages (from-to)2360-2370
JournalJournal of Biomedical Materials Research. Part A
Volume107
Issue number10
DOIs
Publication statusPublished - Oct 2019

Bibliographical note

Export Date: 22 October 2019

CODEN: JBMRC

Funding

We thank L. de Boer for antimicrobial activity testing and Liquan Deng for animal experiments. This study was supported by the funds of ITI project 868_2012, National Natural Science Foundation of China (31800808, 81470724, 81400475, and 81600844), Natural Science Foundation of Zhejiang Province, China (LY14H140002 and LY17H140010), Guangdong Provincial Science and Technology Plan Project of China (2017A020211026), Cultivation and innovation fund of Jinan University (21617312), and Natural Science Foundation of Hebei Province, China (H2017209238). Cultivation and innovation fund of Jinan University, Grant/Award Number: 21617312; Guangdong Provincial Science and Technology Plan Project of China, Grant/Award Number: 2017A020211026; National Natural Science Foundation of China, Grant/Award Number: 81470724 81400475 81600844 31800808; Natural Science Foundation of Hebei Province, China, Grant/Award Number: H2017209238; Natural Science Foundation of Zhejiang Province, China, Grant/Award Number: LY14H140002 LY17H140010; the funds of ITI project, Grant/Award Number: 868_2012

FundersFunder number
Cultivation and innovation fund of Jinan University
Guangdong Provincial Science and Technology Plan Project of China2017A020211026
Natural Science Foundation of Hebei Province, China
National Natural Science Foundation of China31800808, 81600844, 81470724, 81470724 81400475 81600844 31800808, 81400475
Natural Science Foundation of Hebei ProvinceH2017209238
Jinan University21617312
Natural Science Foundation of Zhejiang ProvinceLY14H140002, LY14H140002 LY17H140010, LY17H140010
International Team for Implantology868_2012

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