A Femtosecond Visible/Visible and Visible/Mid-Infrared Transient Absorption Study of the Light Harvesting Complex II

A.D. Stahl; M. Di Donato; I.H.M. van Stokkum; R. van Grondelle; M.L. Groot

doi:10.1016/j.bpj.2009.09.037

A Femtosecond Visible/Visible and Visible/Mid-Infrared Transient Absorption Study of the Light Harvesting Complex II

A.D. Stahl, M. Di Donato, I.H.M. van Stokkum, R. van Grondelle, M.L. Groot

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Light harvesting complex II (LHCII) is the most abundant protein in the thylakoid membrane of higher plants and green algae. LHCII acts to collect solar radiation, transferring this energy mainly toward photosystem II, with a smaller amount going to photosystem I; it is then converted into a chemical, storable form. We performed time-resolved femtosecond visible pump/mid-infrared probe and visible pump/visible probe absorption difference spectroscopy on purified LHCII to gain insight into the energy transfer in this complex occurring in the femto-picosecond time regime. We find that information derived from mid-infrared spectra, together with structural and modeling information, provides a unique visualization of the flow of energy via the bottleneck pigment chlorophyll a604. © 2009 by the Biophysical Society.

Original language	English
Pages (from-to)	3215-3223
Journal	Biophysical Journal
Volume	97
Issue number	12
DOIs	https://doi.org/10.1016/j.bpj.2009.09.037
Publication status	Published - 2009

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title = "A Femtosecond Visible/Visible and Visible/Mid-Infrared Transient Absorption Study of the Light Harvesting Complex II",

abstract = "Light harvesting complex II (LHCII) is the most abundant protein in the thylakoid membrane of higher plants and green algae. LHCII acts to collect solar radiation, transferring this energy mainly toward photosystem II, with a smaller amount going to photosystem I; it is then converted into a chemical, storable form. We performed time-resolved femtosecond visible pump/mid-infrared probe and visible pump/visible probe absorption difference spectroscopy on purified LHCII to gain insight into the energy transfer in this complex occurring in the femto-picosecond time regime. We find that information derived from mid-infrared spectra, together with structural and modeling information, provides a unique visualization of the flow of energy via the bottleneck pigment chlorophyll a604. {\textcopyright} 2009 by the Biophysical Society.",

author = "A.D. Stahl and {Di Donato}, M. and {van Stokkum}, I.H.M. and {van Grondelle}, R. and M.L. Groot",

year = "2009",

doi = "10.1016/j.bpj.2009.09.037",

language = "English",

volume = "97",

pages = "3215--3223",

journal = "Biophysical Journal",

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T1 - A Femtosecond Visible/Visible and Visible/Mid-Infrared Transient Absorption Study of the Light Harvesting Complex II

AU - Stahl, A.D.

AU - Di Donato, M.

AU - van Stokkum, I.H.M.

AU - van Grondelle, R.

AU - Groot, M.L.

PY - 2009

Y1 - 2009

N2 - Light harvesting complex II (LHCII) is the most abundant protein in the thylakoid membrane of higher plants and green algae. LHCII acts to collect solar radiation, transferring this energy mainly toward photosystem II, with a smaller amount going to photosystem I; it is then converted into a chemical, storable form. We performed time-resolved femtosecond visible pump/mid-infrared probe and visible pump/visible probe absorption difference spectroscopy on purified LHCII to gain insight into the energy transfer in this complex occurring in the femto-picosecond time regime. We find that information derived from mid-infrared spectra, together with structural and modeling information, provides a unique visualization of the flow of energy via the bottleneck pigment chlorophyll a604. © 2009 by the Biophysical Society.

AB - Light harvesting complex II (LHCII) is the most abundant protein in the thylakoid membrane of higher plants and green algae. LHCII acts to collect solar radiation, transferring this energy mainly toward photosystem II, with a smaller amount going to photosystem I; it is then converted into a chemical, storable form. We performed time-resolved femtosecond visible pump/mid-infrared probe and visible pump/visible probe absorption difference spectroscopy on purified LHCII to gain insight into the energy transfer in this complex occurring in the femto-picosecond time regime. We find that information derived from mid-infrared spectra, together with structural and modeling information, provides a unique visualization of the flow of energy via the bottleneck pigment chlorophyll a604. © 2009 by the Biophysical Society.

U2 - 10.1016/j.bpj.2009.09.037

DO - 10.1016/j.bpj.2009.09.037

M3 - Article

SN - 0006-3495

VL - 97

SP - 3215

EP - 3223

JO - Biophysical Journal

JF - Biophysical Journal

IS - 12

ER -

A Femtosecond Visible/Visible and Visible/Mid-Infrared Transient Absorption Study of the Light Harvesting Complex II

Abstract

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