Abstract
While Vibrio splendidus is best known as an opportunistic pathogen in oysters, Vibrio splendidus strain 1A01 was first identified as an early colonizer of synthetic chitin particles incubated in seawater. To gain a better understanding of its metabolism, a genome-scale metabolic model (GSMM) of V. splendidus 1A01 was reconstructed. GSMMs enable us to simulate all metabolic reactions in a bacterial cell using flux balance analysis. A draft model was built using an automated pipeline from BioCyc. Manual curation was then performed based on experimental data, in part by gap-filling metabolic pathways and tailoring the model’s biomass reaction to V. splendidus 1A01. The challenges of building a metabolic model for a marine microorganism like V. splendidus 1A01 are described.
| Original language | English |
|---|---|
| Pages (from-to) | 1-15 |
| Number of pages | 15 |
| Journal | mSystems |
| Volume | 8 |
| Issue number | 2 |
| Early online date | 28 Feb 2023 |
| DOIs | |
| Publication status | Published - Apr 2023 |
Bibliographical note
Funding Information:This work was supported by the Simons Foundation through the Principles of Microbial Ecosystems (PriME) collaboration (grant no. 542381 to S.B., 542387 to T.H., and 542395 to O.X.C.).
Publisher Copyright:
© 2023 Iffland-Stettner et al.
Funding
This work was supported by the Simons Foundation through the Principles of Microbial Ecosystems (PriME) collaboration (grant no. 542381 to S.B., 542387 to T.H., and 542395 to O.X.C.).
Keywords
- metabolic modeling
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