TY - JOUR
T1 - A high intake of industrial or ruminant trans fatty acids does not affect the plasma proteome in healthy men
AU - Roos, B.
AU - Wanders, A.J.
AU - Wood, S.
AU - Horgan, G
AU - Rucklige, G.
AU - Reid, M.
AU - Siebelink, E.
AU - Brouwer, I.A.
PY - 2011
Y1 - 2011
N2 - Consumption of industrial trans fat raises the risk of cardiovascular disease, but it is unclear whether cis9,trans11-conjugated linoleic acid (CLA) - a trans fatty acid in dairy products - modulates disease development. We investigated the effects of complete diets providing 7% of energy as industrial trans fat or cis9, trans11 CLA, compared with oleic acid, on regulation of plasma proteins in 12 healthy men. Diets were provided for 3wk each, in random order. Plasma was collected at the end of each 3 wk intervention period, depleted of its 12 most abundant proteins and analyzed by 2-DE. Principal component analysis of protein spot intensity values revealed that the nature of the dietary intervention did not significantly affect the plasma proteome. The intervention provided in the 1st period produced a significant treatment effect compared with the interventions provided in the other two periods, and there was a significant subject effect. In conclusion, the nature of an extreme dietary intervention, i.e. 7% of energy provided by industrial trans fat or cis9,trans11 CLA, did not markedly affect the plasma proteome. Thus plasma proteomics using 2-DE appears, by and large, an unsuitable approach to detect regulation of plasma proteins due to changes in the diet. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - Consumption of industrial trans fat raises the risk of cardiovascular disease, but it is unclear whether cis9,trans11-conjugated linoleic acid (CLA) - a trans fatty acid in dairy products - modulates disease development. We investigated the effects of complete diets providing 7% of energy as industrial trans fat or cis9, trans11 CLA, compared with oleic acid, on regulation of plasma proteins in 12 healthy men. Diets were provided for 3wk each, in random order. Plasma was collected at the end of each 3 wk intervention period, depleted of its 12 most abundant proteins and analyzed by 2-DE. Principal component analysis of protein spot intensity values revealed that the nature of the dietary intervention did not significantly affect the plasma proteome. The intervention provided in the 1st period produced a significant treatment effect compared with the interventions provided in the other two periods, and there was a significant subject effect. In conclusion, the nature of an extreme dietary intervention, i.e. 7% of energy provided by industrial trans fat or cis9,trans11 CLA, did not markedly affect the plasma proteome. Thus plasma proteomics using 2-DE appears, by and large, an unsuitable approach to detect regulation of plasma proteins due to changes in the diet. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
U2 - 10.1002/pmic.201100163
DO - 10.1002/pmic.201100163
M3 - Article
VL - 11
SP - 3928
EP - 3934
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 19
ER -