A laser microdissection–liquid chromatography–tandem mass spectrometry workflow for post-mortem analysis of brain tissue

David C. Hondius*, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, Ka Wan Li, August B. Smit

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Improved speed and sensitivity of mass spectrometry allow the simultaneous quantification of high numbers of proteins from increasingly smaller quantities of tissue sample. Quantitative data of the proteome is highly valuable for providing unbiased information on, for example, protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analyzing this type of samples is laser micro-dissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe different options for isolating tissue suitable for LC-MS/MS analysis.

Original languageEnglish
Title of host publicationLaser Capture Microdissection
Subtitle of host publicationMethods and Protocols
Place of PublicationNew York
PublisherHumana Press Inc
Pages371-383
Number of pages13
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1723
ISSN (Print)1064-3745

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Keywords

  • Human brain
  • Immunohistochemistry
  • Inclusion bodies
  • Laser capture
  • Laser microdissection
  • Mass spectrometry
  • Post-mortem tissue
  • Protein aggregates
  • Proteomics
  • Single cell

Cite this

Hondius, D. C., Hoozemans, J. J. M., Rozemuller, A. J. M., Li, K. W., & Smit, A. B. (2018). A laser microdissection–liquid chromatography–tandem mass spectrometry workflow for post-mortem analysis of brain tissue. In Laser Capture Microdissection: Methods and Protocols (pp. 371-383). (Methods in Molecular Biology; Vol. 1723). New York: Humana Press Inc. https://doi.org/10.1007/978-1-4939-7558-7_21