A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles

Lianzhen Li, Huifeng Wu, Chenglong Ji, C.A.M. van Gestel, H.E. Allen, W.J.G.M. Peijnenburg

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

We examined the short-term toxicity of AgNPs and AgNO<inf>3</inf> to Daphnia magna at sublethal levels using 1H NMR-based metabolomics. Two sizes of polyvinylpyrrolidone-coated AgNPs (10 and 40nm) were synthesized and characterized and their Ag+ release was studied using centrifugal ultrafiltration and inductively coupled plasma mass spectrometry. Multivariate statistical analysis of the 1H NMR spectra showed significant changes in the D. magna metabolic profiles following 48h exposure to both AgNP particle sizes and Ag+ exposure. Most of the metabolic biomarkers for AgNP exposure, including 3-hydroxybutyrate, arginine, lysine and phosphocholine, were identical to those of the Ag+-exposed groups, suggesting that the dominant effects of both AgNPs were due to released Ag+. The observed metabolic changes implied that the released Ag+ induced disturbance in energy metabolism and oxidative stress, a proposed mechanism of AgNP toxicity. Elevated levels of lactate in all AgNP-treated but not in Ag+-treated groups provided evidence for Ag-NP enhanced anaerobic metabolism. These findings show that 1H NMR-based metabolomics provides a sensitive measure of D. magna response to AgNPs and that further targeted assays are needed to elucidate mechanisms of action of nanoparticle-induced toxicity.
Original languageEnglish
Pages (from-to)66-73
JournalEcotoxicology and Environmental Safety
Volume119
DOIs
Publication statusPublished - 2015

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Daphnia
Silver Nitrate
Metabolomics
Silver
Nanoparticles
Toxicity
Nitrates
Nuclear magnetic resonance
Anaerobiosis
Povidone
Inductively coupled plasma mass spectrometry
Arginine
Oxidative stress
Phosphorylcholine
3-Hydroxybutyric Acid
Metabolome
Ultrafiltration
Biomarkers
Particle Size
Metabolism

Cite this

Li, Lianzhen ; Wu, Huifeng ; Ji, Chenglong ; van Gestel, C.A.M. ; Allen, H.E. ; Peijnenburg, W.J.G.M. / A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles. In: Ecotoxicology and Environmental Safety. 2015 ; Vol. 119. pp. 66-73.
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abstract = "We examined the short-term toxicity of AgNPs and AgNO3 to Daphnia magna at sublethal levels using 1H NMR-based metabolomics. Two sizes of polyvinylpyrrolidone-coated AgNPs (10 and 40nm) were synthesized and characterized and their Ag+ release was studied using centrifugal ultrafiltration and inductively coupled plasma mass spectrometry. Multivariate statistical analysis of the 1H NMR spectra showed significant changes in the D. magna metabolic profiles following 48h exposure to both AgNP particle sizes and Ag+ exposure. Most of the metabolic biomarkers for AgNP exposure, including 3-hydroxybutyrate, arginine, lysine and phosphocholine, were identical to those of the Ag+-exposed groups, suggesting that the dominant effects of both AgNPs were due to released Ag+. The observed metabolic changes implied that the released Ag+ induced disturbance in energy metabolism and oxidative stress, a proposed mechanism of AgNP toxicity. Elevated levels of lactate in all AgNP-treated but not in Ag+-treated groups provided evidence for Ag-NP enhanced anaerobic metabolism. These findings show that 1H NMR-based metabolomics provides a sensitive measure of D. magna response to AgNPs and that further targeted assays are needed to elucidate mechanisms of action of nanoparticle-induced toxicity.",
author = "Lianzhen Li and Huifeng Wu and Chenglong Ji and {van Gestel}, C.A.M. and H.E. Allen and W.J.G.M. Peijnenburg",
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A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles. / Li, Lianzhen; Wu, Huifeng; Ji, Chenglong; van Gestel, C.A.M.; Allen, H.E.; Peijnenburg, W.J.G.M.

In: Ecotoxicology and Environmental Safety, Vol. 119, 2015, p. 66-73.

Research output: Contribution to JournalArticleAcademicpeer-review

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