TY - JOUR
T1 - A method for nitric oxide radical scavenging properties of sulfur containing compounds.
AU - Vriesman, M.F.
AU - Haenen, G.R.M.M.
AU - Westerveld, G.J.
AU - Paquay, J.B.G.
AU - Voss, H.P.
AU - Bast, A.
PY - 1997
Y1 - 1997
N2 - A new method for the quantification of the nitric oxide (NO) scavenging activity of compounds in aqueous solutions is described using an amperometric NO sensor. After correction for the spontaneous degradation of NO, second-order rate kinetics of the scavenging reaction are observed. The rate constant for haemoglobin found with this method is comparable with that found with an established spectrophotometric method. To demonstrate the capability of the method, several sulfur-containing compounds were tested (GSH, GSSG, S-methyl glutathione, N-acetyl cysteine, lipoic acid and dihydrolipoic acid). Of these compounds, only those that contained a thiol group displayed a considerable NO scavenging activity.
AB - A new method for the quantification of the nitric oxide (NO) scavenging activity of compounds in aqueous solutions is described using an amperometric NO sensor. After correction for the spontaneous degradation of NO, second-order rate kinetics of the scavenging reaction are observed. The rate constant for haemoglobin found with this method is comparable with that found with an established spectrophotometric method. To demonstrate the capability of the method, several sulfur-containing compounds were tested (GSH, GSSG, S-methyl glutathione, N-acetyl cysteine, lipoic acid and dihydrolipoic acid). Of these compounds, only those that contained a thiol group displayed a considerable NO scavenging activity.
U2 - 10.1023/A:1008601327920
DO - 10.1023/A:1008601327920
M3 - Article
VL - 19
SP - 283
EP - 286
JO - Pharmacy World and Science
JF - Pharmacy World and Science
SN - 0928-1231
ER -
