A method for the determination of volatile ammonia in air, using a nitrogen-cooled trap and fluorometric detection

H.G. Westra; R.G. Tigchelaar; J.A. Berden

doi:10.1006/abio.2001.5229

A method for the determination of volatile ammonia in air, using a nitrogen-cooled trap and fluorometric detection

H.G. Westra, R.G. Tigchelaar, J.A. Berden

Human Movement Sciences

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A quick, cheap, and accurate method for the determination of ammonia in air is described. Ammonia and water vapor are trapped simultaneously in a gas sampling tube cooled in liquid nitrogen. Subsequently ammonia is derivatized with o-phthaldialdehyde and determined using fluorescence detection. The detection limit of ammonia in a gaseous sample is about 1 nmol per liter of gas. The recovery, using a calibration gas of 6.00 ppm ammonia in nitrogen, is 102.9 ± 6.4%. Examples are presented in which this method is used for the determination of ammonia in environmental air and in expired air during exhaustive exercise of a human subject. It is suggested that this method can be used for the determination of volatile ammonia and other compounds in air during environmental and biological monitoring and in research. © 2001 Academic Press.

Original language	English
Pages (from-to)	225-231
Journal	Analytical Biochemistry
Volume	296
DOIs	https://doi.org/10.1006/abio.2001.5229
Publication status	Published - 2001

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verschenen in mei 2002

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title = "A method for the determination of volatile ammonia in air, using a nitrogen-cooled trap and fluorometric detection",

abstract = "A quick, cheap, and accurate method for the determination of ammonia in air is described. Ammonia and water vapor are trapped simultaneously in a gas sampling tube cooled in liquid nitrogen. Subsequently ammonia is derivatized with o-phthaldialdehyde and determined using fluorescence detection. The detection limit of ammonia in a gaseous sample is about 1 nmol per liter of gas. The recovery, using a calibration gas of 6.00 ppm ammonia in nitrogen, is 102.9 ± 6.4%. Examples are presented in which this method is used for the determination of ammonia in environmental air and in expired air during exhaustive exercise of a human subject. It is suggested that this method can be used for the determination of volatile ammonia and other compounds in air during environmental and biological monitoring and in research. {\textcopyright} 2001 Academic Press.",

author = "H.G. Westra and R.G. Tigchelaar and J.A. Berden",

note = "verschenen in mei 2002",

year = "2001",

doi = "10.1006/abio.2001.5229",

language = "English",

volume = "296",

pages = "225--231",

journal = "Analytical Biochemistry",

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T1 - A method for the determination of volatile ammonia in air, using a nitrogen-cooled trap and fluorometric detection

AU - Westra, H.G.

AU - Tigchelaar, R.G.

AU - Berden, J.A.

N1 - verschenen in mei 2002

PY - 2001

Y1 - 2001

N2 - A quick, cheap, and accurate method for the determination of ammonia in air is described. Ammonia and water vapor are trapped simultaneously in a gas sampling tube cooled in liquid nitrogen. Subsequently ammonia is derivatized with o-phthaldialdehyde and determined using fluorescence detection. The detection limit of ammonia in a gaseous sample is about 1 nmol per liter of gas. The recovery, using a calibration gas of 6.00 ppm ammonia in nitrogen, is 102.9 ± 6.4%. Examples are presented in which this method is used for the determination of ammonia in environmental air and in expired air during exhaustive exercise of a human subject. It is suggested that this method can be used for the determination of volatile ammonia and other compounds in air during environmental and biological monitoring and in research. © 2001 Academic Press.

AB - A quick, cheap, and accurate method for the determination of ammonia in air is described. Ammonia and water vapor are trapped simultaneously in a gas sampling tube cooled in liquid nitrogen. Subsequently ammonia is derivatized with o-phthaldialdehyde and determined using fluorescence detection. The detection limit of ammonia in a gaseous sample is about 1 nmol per liter of gas. The recovery, using a calibration gas of 6.00 ppm ammonia in nitrogen, is 102.9 ± 6.4%. Examples are presented in which this method is used for the determination of ammonia in environmental air and in expired air during exhaustive exercise of a human subject. It is suggested that this method can be used for the determination of volatile ammonia and other compounds in air during environmental and biological monitoring and in research. © 2001 Academic Press.

U2 - 10.1006/abio.2001.5229

DO - 10.1006/abio.2001.5229

M3 - Article

VL - 296

SP - 225

EP - 231

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

ER -

A method for the determination of volatile ammonia in air, using a nitrogen-cooled trap and fluorometric detection

Abstract

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