A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles

Linglei Jiang; Joen Luirink; Sander A.A. Kooijmans; Kok P.M. van Kessel; Wouter Jong; Max van Essen; Cor W. Seinen; Steven de Maat; Olivier G. de Jong; Jerney F.F. Gitz-François; Wim E. Hennink; Pieter Vader; Raymond M. Schiffelers

doi:10.1016/j.bbagen.2020.129763

A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles

Linglei Jiang, Joen Luirink, Sander A.A. Kooijmans, Kok P.M. van Kessel, Wouter Jong, Max van Essen, Cor W. Seinen, Steven de Maat, Olivier G. de Jong, Jerney F.F. Gitz-François, Wim E. Hennink, Pieter Vader, Raymond M. Schiffelers^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a ‘post-insertion’ approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.

Original language	English
Article number	129763
Pages (from-to)	1-9
Number of pages	9
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1865
Issue number	4
Early online date	14 Oct 2020
DOIs	https://doi.org/10.1016/j.bbagen.2020.129763
Publication status	Published - Apr 2021

Keywords

Extracellular vesicles
Outer membrane vesicles
Poly(ethylene glycol)lipids
Post-insertion

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Jiang, L., Luirink, J., Kooijmans, S. A. A., van Kessel, K. P. M., Jong, W., van Essen, M., Seinen, C. W., de Maat, S., de Jong, O. G., Gitz-François, J. F. F., Hennink, W. E., Vader, P., & Schiffelers, R. M. (2021). A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles. Biochimica et Biophysica Acta - General Subjects, 1865(4), 1-9. [129763]. https://doi.org/10.1016/j.bbagen.2020.129763

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abstract = "Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a {\textquoteleft}post-insertion{\textquoteright} approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.",

keywords = "Extracellular vesicles, Outer membrane vesicles, Poly(ethylene glycol)lipids, Post-insertion",

author = "Linglei Jiang and Joen Luirink and Kooijmans, {Sander A.A.} and {van Kessel}, {Kok P.M.} and Wouter Jong and {van Essen}, Max and Seinen, {Cor W.} and {de Maat}, Steven and {de Jong}, {Olivier G.} and Gitz-Fran{\c c}ois, {Jerney F.F.} and Hennink, {Wim E.} and Pieter Vader and Schiffelers, {Raymond M.}",

year = "2021",

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doi = "10.1016/j.bbagen.2020.129763",

language = "English",

volume = "1865",

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Jiang, L, Luirink, J, Kooijmans, SAA, van Kessel, KPM, Jong, W, van Essen, M, Seinen, CW, de Maat, S, de Jong, OG, Gitz-François, JFF, Hennink, WE, Vader, P & Schiffelers, RM 2021, 'A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles', Biochimica et Biophysica Acta - General Subjects, vol. 1865, no. 4, 129763, pp. 1-9. https://doi.org/10.1016/j.bbagen.2020.129763

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AU - Jiang, Linglei

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AU - Kooijmans, Sander A.A.

AU - van Kessel, Kok P.M.

AU - Jong, Wouter

AU - van Essen, Max

AU - Seinen, Cor W.

AU - de Maat, Steven

AU - de Jong, Olivier G.

AU - Gitz-François, Jerney F.F.

AU - Hennink, Wim E.

AU - Vader, Pieter

AU - Schiffelers, Raymond M.

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AB - Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a ‘post-insertion’ approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.

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A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles

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Keywords

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