A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles

Linglei Jiang, Joen Luirink, Sander A.A. Kooijmans, Kok P.M. van Kessel, Wouter Jong, Max van Essen, Cor W. Seinen, Steven de Maat, Olivier G. de Jong, Jerney F.F. Gitz-François, Wim E. Hennink, Pieter Vader, Raymond M. Schiffelers*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a ‘post-insertion’ approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.

Original languageEnglish
Article number129763
JournalBiochimica et Biophysica Acta - General Subjects
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Extracellular vesicles
  • Outer membrane vesicles
  • Poly(ethylene glycol)lipids
  • Post-insertion

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