A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes

Cormac Murphy; Elisabeth Feifel; Paul Jennings; Gerhard Gstraunthaler; Anja Wilmes

doi:10.1007/978-1-4939-9477-9_8

A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes

Cormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, Anja Wilmes^*

^*Corresponding author for this work

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Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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Abstract

Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.

Original language	English
Title of host publication	Cell-Based Assays Using iPSCs for Drug Development and Testing
Editors	C.F. Mandenius, J. Ross
Publisher	Humana Press Inc.
Chapter	8
Pages	93-99
Number of pages	7
ISBN (Electronic)	9781493994779
ISBN (Print)	9781493994762, 9781493994793
DOIs	https://doi.org/10.1007/978-1-4939-9477-9_8
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1994
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Differentiation
Human induced pluripotent cells
Kidney
Pericytes
Podocyte

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10.1007/978-1-4939-9477-9_8

A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytesFinal published version, 767 KBLicence: Article 25fa Dutch Copyright Act

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Murphy, C., Feifel, E., Jennings, P., Gstraunthaler, G., & Wilmes, A. (2019). A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. In C. F. Mandenius, & J. Ross (Eds.), Cell-Based Assays Using iPSCs for Drug Development and Testing (pp. 93-99). (Methods in Molecular Biology; Vol. 1994). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9477-9_8

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Murphy, C, Feifel, E, Jennings, P, Gstraunthaler, G & Wilmes, A 2019, A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. in CF Mandenius & J Ross (eds), Cell-Based Assays Using iPSCs for Drug Development and Testing. Methods in Molecular Biology, vol. 1994, Humana Press Inc., pp. 93-99. https://doi.org/10.1007/978-1-4939-9477-9_8

A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. / Murphy, Cormac; Feifel, Elisabeth; Jennings, Paul et al.
Cell-Based Assays Using iPSCs for Drug Development and Testing. ed. / C.F. Mandenius; J. Ross. Humana Press Inc., 2019. p. 93-99 (Methods in Molecular Biology; Vol. 1994).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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AB - Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.

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Murphy C, Feifel E, Jennings P, Gstraunthaler G, Wilmes A. A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. In Mandenius CF, Ross J, editors, Cell-Based Assays Using iPSCs for Drug Development and Testing. Humana Press Inc. 2019. p. 93-99. (Methods in Molecular Biology). Epub 2019 May 24. doi: 10.1007/978-1-4939-9477-9_8

A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes

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