Abstract
Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.
| Original language | English |
|---|---|
| Title of host publication | Cell-Based Assays Using iPSCs for Drug Development and Testing |
| Editors | C.F. Mandenius, J. Ross |
| Publisher | Humana Press Inc. |
| Pages | 93-99 |
| Number of pages | 7 |
| Volume | 1994 |
| ISBN (Electronic) | 9781493994779 |
| ISBN (Print) | 9781493994762 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1994 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Differentiation
- Human induced pluripotent cells
- Kidney
- Pericytes
- Podocyte
Cite this
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A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. / Murphy, Cormac; Feifel, Elisabeth; Jennings, Paul; Gstraunthaler, Gerhard; Wilmes, Anja.
Cell-Based Assays Using iPSCs for Drug Development and Testing. ed. / C.F. Mandenius; J. Ross. Vol. 1994 Humana Press Inc., 2019. p. 93-99 (Methods in Molecular Biology; Vol. 1994).Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review
TY - CHAP
T1 - A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes
AU - Murphy, Cormac
AU - Feifel, Elisabeth
AU - Jennings, Paul
AU - Gstraunthaler, Gerhard
AU - Wilmes, Anja
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.
AB - Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.
KW - Differentiation
KW - Human induced pluripotent cells
KW - Kidney
KW - Pericytes
KW - Podocyte
UR - http://www.scopus.com/inward/record.url?scp=85066279486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066279486&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9477-9_8
DO - 10.1007/978-1-4939-9477-9_8
M3 - Chapter
SN - 9781493994762
VL - 1994
T3 - Methods in Molecular Biology
SP - 93
EP - 99
BT - Cell-Based Assays Using iPSCs for Drug Development and Testing
A2 - Mandenius, C.F.
A2 - Ross, J.
PB - Humana Press Inc.
ER -