A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes

Cormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, Anja Wilmes

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.

Original languageEnglish
Title of host publicationCell-Based Assays Using iPSCs for Drug Development and Testing
EditorsC.F. Mandenius, J. Ross
PublisherHumana Press Inc.
Pages93-99
Number of pages7
Volume1994
ISBN (Electronic)9781493994779
ISBN (Print)9781493994762
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1994
ISSN (Print)1064-3745

Fingerprint

Induced Pluripotent Stem Cells
Podocytes
Glomerular Filtration Barrier
Cell Culture Techniques
Epithelial Cells

Keywords

  • Differentiation
  • Human induced pluripotent cells
  • Kidney
  • Pericytes
  • Podocyte

Cite this

Murphy, C., Feifel, E., Jennings, P., Gstraunthaler, G., & Wilmes, A. (2019). A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. In C. F. Mandenius, & J. Ross (Eds.), Cell-Based Assays Using iPSCs for Drug Development and Testing (Vol. 1994, pp. 93-99). (Methods in Molecular Biology; Vol. 1994). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9477-9_8
Murphy, Cormac ; Feifel, Elisabeth ; Jennings, Paul ; Gstraunthaler, Gerhard ; Wilmes, Anja. / A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. Cell-Based Assays Using iPSCs for Drug Development and Testing. editor / C.F. Mandenius ; J. Ross. Vol. 1994 Humana Press Inc., 2019. pp. 93-99 (Methods in Molecular Biology).
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Murphy, C, Feifel, E, Jennings, P, Gstraunthaler, G & Wilmes, A 2019, A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. in CF Mandenius & J Ross (eds), Cell-Based Assays Using iPSCs for Drug Development and Testing. vol. 1994, Methods in Molecular Biology, vol. 1994, Humana Press Inc., pp. 93-99. https://doi.org/10.1007/978-1-4939-9477-9_8

A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. / Murphy, Cormac; Feifel, Elisabeth; Jennings, Paul; Gstraunthaler, Gerhard; Wilmes, Anja.

Cell-Based Assays Using iPSCs for Drug Development and Testing. ed. / C.F. Mandenius; J. Ross. Vol. 1994 Humana Press Inc., 2019. p. 93-99 (Methods in Molecular Biology; Vol. 1994).

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

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Murphy C, Feifel E, Jennings P, Gstraunthaler G, Wilmes A. A protocol for one-step differentiation of human induced pluripotent stem cells into mature podocytes. In Mandenius CF, Ross J, editors, Cell-Based Assays Using iPSCs for Drug Development and Testing. Vol. 1994. Humana Press Inc. 2019. p. 93-99. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9477-9_8