A role for alpha 11 beta 1 integrin in the human periodontal ligament

M.M. Barczyk; L.H.B. Olsen; P. da Franca; B.G. Loos; K. Mustafa; D. Gullberg; A.I. Bolstad

doi:10.1177/0022034509339291

A role for alpha 11 beta 1 integrin in the human periodontal ligament

M.M. Barczyk, L.H.B. Olsen, P. da Franca, B.G. Loos, K. Mustafa, D. Gullberg, A.I. Bolstad

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

We previously demonstrated a role for α11β1 integrin in periodontal ligament (PDL)-driven tooth eruption in the mouse. To explore a possible role for α11β1 in the human periodontium, we have characterized the expression and function of α11 in human PDL tissue, in human PDL fibroblasts (hPDLF), and in human gingival fibroblasts (hGF). α11 expression was detected in PDL tissue, in hPDLF, and in hGF cells. Platelet-derived growth factor-BB and insulin-like growth factor II stimulated contraction of collagen lattices by both types of fibroblasts. α2 integrin blocking antibodies and the use of α11 siRNA demonstrated a role for both α2β1 and α11β1 in collagen lattice remodeling. Analysis of the proximal ITGA11 promoter from persons with chronic periodontal disease failed to reveal any polymorphism. Analysis of our data shows that α11β1 is a major collagen receptor on cultured human PDL cells and implies that it is also functionally important in the PDL in vivo.

Original language	Undefined/Unknown
Pages (from-to)	621-626
Journal	Journal of Dental Research
Volume	88
Issue number	7
DOIs	https://doi.org/10.1177/0022034509339291
Publication status	Published - 2009

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10.1177/0022034509339291

https://hdl.handle.net/11245/1.313154

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title = "A role for alpha 11 beta 1 integrin in the human periodontal ligament",

abstract = "We previously demonstrated a role for α11β1 integrin in periodontal ligament (PDL)-driven tooth eruption in the mouse. To explore a possible role for α11β1 in the human periodontium, we have characterized the expression and function of α11 in human PDL tissue, in human PDL fibroblasts (hPDLF), and in human gingival fibroblasts (hGF). α11 expression was detected in PDL tissue, in hPDLF, and in hGF cells. Platelet-derived growth factor-BB and insulin-like growth factor II stimulated contraction of collagen lattices by both types of fibroblasts. α2 integrin blocking antibodies and the use of α11 siRNA demonstrated a role for both α2β1 and α11β1 in collagen lattice remodeling. Analysis of the proximal ITGA11 promoter from persons with chronic periodontal disease failed to reveal any polymorphism. Analysis of our data shows that α11β1 is a major collagen receptor on cultured human PDL cells and implies that it is also functionally important in the PDL in vivo.",

author = "M.M. Barczyk and L.H.B. Olsen and {da Franca}, P. and B.G. Loos and K. Mustafa and D. Gullberg and A.I. Bolstad",

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T1 - A role for alpha 11 beta 1 integrin in the human periodontal ligament

AU - Barczyk, M.M.

AU - Olsen, L.H.B.

AU - da Franca, P.

AU - Loos, B.G.

AU - Mustafa, K.

AU - Gullberg, D.

AU - Bolstad, A.I.

PY - 2009

Y1 - 2009

N2 - We previously demonstrated a role for α11β1 integrin in periodontal ligament (PDL)-driven tooth eruption in the mouse. To explore a possible role for α11β1 in the human periodontium, we have characterized the expression and function of α11 in human PDL tissue, in human PDL fibroblasts (hPDLF), and in human gingival fibroblasts (hGF). α11 expression was detected in PDL tissue, in hPDLF, and in hGF cells. Platelet-derived growth factor-BB and insulin-like growth factor II stimulated contraction of collagen lattices by both types of fibroblasts. α2 integrin blocking antibodies and the use of α11 siRNA demonstrated a role for both α2β1 and α11β1 in collagen lattice remodeling. Analysis of the proximal ITGA11 promoter from persons with chronic periodontal disease failed to reveal any polymorphism. Analysis of our data shows that α11β1 is a major collagen receptor on cultured human PDL cells and implies that it is also functionally important in the PDL in vivo.

AB - We previously demonstrated a role for α11β1 integrin in periodontal ligament (PDL)-driven tooth eruption in the mouse. To explore a possible role for α11β1 in the human periodontium, we have characterized the expression and function of α11 in human PDL tissue, in human PDL fibroblasts (hPDLF), and in human gingival fibroblasts (hGF). α11 expression was detected in PDL tissue, in hPDLF, and in hGF cells. Platelet-derived growth factor-BB and insulin-like growth factor II stimulated contraction of collagen lattices by both types of fibroblasts. α2 integrin blocking antibodies and the use of α11 siRNA demonstrated a role for both α2β1 and α11β1 in collagen lattice remodeling. Analysis of the proximal ITGA11 promoter from persons with chronic periodontal disease failed to reveal any polymorphism. Analysis of our data shows that α11β1 is a major collagen receptor on cultured human PDL cells and implies that it is also functionally important in the PDL in vivo.

U2 - 10.1177/0022034509339291

DO - 10.1177/0022034509339291

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