TY - JOUR
T1 - A routine method to determine the chlorophyll a, pheophytin a and B-carotene contents of isolated photosystem II reaction center complexes.
AU - Eijckelhoff, C.
AU - Dekker, J.P.
PY - 1997
Y1 - 1997
N2 - The most simple way in which the stoichiometry of chlorophyll a, pheophytin a and ̄-carotene in isolated Photosystem II reaction center complexes can be determined is by analysis of the spectrum of the extracted pigments in 80% acetone. We present two different calculation methods using the extinction coefficients of the purified pigments in 80% acetone at different wavelengths. One of these methods also accounts for the possible presence of chlorophyll b. The results are compared with results obtained with HPLC pigment analysis, and indicate that these methods are suitable for routine determination of the pigment stoichiometry of isolated Photosystem II reaction center complexes.
AB - The most simple way in which the stoichiometry of chlorophyll a, pheophytin a and ̄-carotene in isolated Photosystem II reaction center complexes can be determined is by analysis of the spectrum of the extracted pigments in 80% acetone. We present two different calculation methods using the extinction coefficients of the purified pigments in 80% acetone at different wavelengths. One of these methods also accounts for the possible presence of chlorophyll b. The results are compared with results obtained with HPLC pigment analysis, and indicate that these methods are suitable for routine determination of the pigment stoichiometry of isolated Photosystem II reaction center complexes.
UR - https://www.scopus.com/pages/publications/0030808379
UR - https://www.scopus.com/inward/citedby.url?scp=0030808379&partnerID=8YFLogxK
U2 - 10.1023/A:1005834006985
DO - 10.1023/A:1005834006985
M3 - Article
SN - 0166-8595
VL - 52
SP - 69
EP - 73
JO - Photosynthesis Research
JF - Photosynthesis Research
ER -
