A second gene at the tomato of Cf-4 locus confers resistance to Cladosporium fulvum through recognition of a novel avirulence determinant.

F.L.W. Takken, C.M. Thomas, M.H.A.J. Joosten, C. Golstein, N. Westerink, J. Hille, H.J.J. Nijkamp, P.J.G.M. de Wit, J.D.G. Jones

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    Abstract

    The tomato Cf-4 and Cf-9 genes confer resistance to the leaf mould pathogen Cladosporium fulvum and map at a complex locus on the short arm of chromosome 1. It was previously shown that the gene encoding Cf-4, which recognizes the Avr4 avirulence determinant, is one of five tandemly duplicated homologous genes (Hcr9-4s) at this locus. Cf-4 was identified by molecular analysis of rare Cf-4/Cf-9 disease-sensitive recombinants and by complementation analysis. The analysis did not exclude the possibility that an additional gene(s) located distal to Cf-4 may also confer resistance to C. fulvum. We demonstrate that a number of Dissociation-tagged Cf-4 mutants, identified on the basis of their insensitivity to Avr4, are still resistant to infection by C. fulvum race 5. Molecular analysis of 16 Cf-4 mutants, most of which have small chromosomal deletions in this region, suggested the additional resistance specificity is encoded by Hcr9-4E. Hcr9-4E recognizes a novel C. fulvum avirulence determinant that we have designated Avr4E.
    Original languageEnglish
    Pages (from-to)279-288
    Number of pages10
    JournalPlant Journal
    Volume20
    DOIs
    Publication statusPublished - 1999

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    Takken, F. L. W., Thomas, C. M., Joosten, M. H. A. J., Golstein, C., Westerink, N., Hille, J., ... Jones, J. D. G. (1999). A second gene at the tomato of Cf-4 locus confers resistance to Cladosporium fulvum through recognition of a novel avirulence determinant. Plant Journal, 20, 279-288. https://doi.org/10.1046/j.1365-313X.1999.00601.x