A single nucleotide change in the promoter mutp enhances fluoride resistance of Streptococcus mutans

Y. Liao; B.W. Brandt; M. Zhang; J. Li; W. Crielaard; C. van Loveren; D.M. Deng

doi:10.1128/AAC.01366-16

A single nucleotide change in the promoter mutp enhances fluoride resistance of Streptococcus mutans

Y. Liao, B.W. Brandt, M. Zhang, J. Li, W. Crielaard, C. van Loveren, D.M. Deng

Preventive Dentistry

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Abstract

Previously, we identified a single nucleotide mutation in the promoter (mutp) of the fluoride antiporter-coding genes in a naturally fluoride-resistant Streptococcus mutans strain. Here, we studied the role of this mutation in a defined genetic background. The results confirmed that this mutation alone confers fluoride resistance on S. mutans, as shown by growth and lactic acid production assays. This resistance was explained by constitutively higher mutp promoter activity and upregulation of the fluoride antiporter-coding genes.

Original language	English
Pages (from-to)	7509-7512
Journal	Antimicrobial agents and chemotherapy
Volume	60
Issue number	12
Early online date	3 Oct 2016
DOIs	https://doi.org/10.1128/AAC.01366-16
Publication status	Published - Dec 2016

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10.1128/AAC.01366-16

https://hdl.handle.net/11245.1/639047ff-8cbb-4bf5-abb2-474ef4e74fa6

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title = "A single nucleotide change in the promoter mutp enhances fluoride resistance of Streptococcus mutans",

abstract = "Previously, we identified a single nucleotide mutation in the promoter (mutp) of the fluoride antiporter-coding genes in a naturally fluoride-resistant Streptococcus mutans strain. Here, we studied the role of this mutation in a defined genetic background. The results confirmed that this mutation alone confers fluoride resistance on S. mutans, as shown by growth and lactic acid production assays. This resistance was explained by constitutively higher mutp promoter activity and upregulation of the fluoride antiporter-coding genes.",

author = "Y. Liao and B.W. Brandt and M. Zhang and J. Li and W. Crielaard and {van Loveren}, C. and D.M. Deng",

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AU - Brandt, B.W.

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AU - Li, J.

AU - Crielaard, W.

AU - van Loveren, C.

AU - Deng, D.M.

PY - 2016/12

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AB - Previously, we identified a single nucleotide mutation in the promoter (mutp) of the fluoride antiporter-coding genes in a naturally fluoride-resistant Streptococcus mutans strain. Here, we studied the role of this mutation in a defined genetic background. The results confirmed that this mutation alone confers fluoride resistance on S. mutans, as shown by growth and lactic acid production assays. This resistance was explained by constitutively higher mutp promoter activity and upregulation of the fluoride antiporter-coding genes.

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A single nucleotide change in the promoter mutp enhances fluoride resistance of Streptococcus mutans

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