A single-step preparation of carbohydrate functionalized monoliths for separation and trapping of polar compounds

Jincai Wang, Jialiang Guo, Haowen Chen, Xiaoling Huang, Govert W. Somsen, Fenyun Song*, Zhengjin Jiang

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

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A single-step copolymerization strategy was developed for the preparation of carbohydrate (glucose and maltose) functionalized monoliths using click reaction. Firstly, novel carbohydrate-functionalized methacrylate monomers were synthesized through Cu(I)-catalyzed 1,3-dipolar cycloaddition (alkyne-azide reaction) of terminal alkyne with azide of carbohydrate derivatives. The corresponding carbohydrate functionalized monolithic columns were then prepared through a single-step in-situ copolymerization. The physicochemical properties and performance of the fabricated monolithic columns were evaluated using scanning electron microscopy, Fourier-transform infrared spectroscopy, and nano-liquid chromatography. For the optimized monolithic column, satisfactory column permeability and good separation performance were demonstrated for polar compounds including nucleoside, phenolic compounds and benzoic acid derivatives. The monolithic column is also highly useful for selective and efficient enrichment of glycopeptides from human IgG tryptic digests. This study not only provided a novel hydrophilic column for separation and selective trapping of polar compounds, but also proposed a facile and efficient approach for preparing carbohydrate functionalized monoliths.

Original languageEnglish
Article number461481
Pages (from-to)1-7
Number of pages7
JournalJournal of Chromatography A
Early online date16 Aug 2020
Publication statusPublished - 27 Sept 2020


  • Carbohydrate functionalized monolith
  • Click chemistry
  • Single-step preparation


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