A specific binding site for neoxanthin in the monomeric antenna proteins CP26 and CP29 of Photosystem II

Stefano Caffarri, Francesca Passarini, Roberto Bassi, Roberta Croce*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The location of the neoxanthin binding site in CP26 and CP29 was investigated by site-directed mutagenesis. The crystallographic structure of LHCII shows that the binding of neoxanthin to the N1 site is stabilised by an H bond with a tyrosine in the lumenal loop. This residue is conserved in CP26 and CP29. Mutation of this tyrosine into phenylalanine induced specific loss of neoxanthin without affecting violaxanthin binding. In contrast to previous proposals, it is thus concluded that also in these minor antenna complexes neoxanthin is accommodated in the N1 site. The characteristics of this binding site in the different antenna complexes are discussed.

Original languageEnglish
Pages (from-to)4704-4710
Number of pages7
JournalFEBS Letters
Volume581
Issue number24
DOIs
Publication statusPublished - 2 Oct 2007

Keywords

  • CP26
  • CP29
  • Light-harvesting complex
  • Neoxanthin
  • Photosynthesis
  • Photosystem II

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