A yeast based screening method for Arabidopsis zearalenone-metabolism mutants

Juan Antonio Torres Acosta*, Ulrike Werner, Herwig Bachmann, Franz Berthiller, Rainer Schuhmacher, Rudolf Krska, Marie Theres Hauser, Gerhard Adam

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review


Arabidopsis thaliana and other plants are able to rapidly metabolize the estrogenic Fusarium metabolite zearalenone (ZON) into ZON-glucoside and further unknown compounds. We present here a rapid and efficient method to screen for Arabidopsis mutants unable to metabolize ZON. Arabidopsis seedling are grown in 96 well microtiter plates and subsequently incubated with ZON and β-glucosidase. The remaining ZON in the supernatant is detected with an engineered estrogen-responsive yeast strain. Extracellular ZON-4- O-glucoside (ZON-Glc), which is not detected by the indicator strain, is converted back to ZON by the added almond β-glucosidase. Wildtype Arabidopsis seedlings inactivate 2 ppm ZON in three days, which is easily detected by the colorimetric readout. The assay should be useful to identify mutants affected in ZON metabolism.

Original languageEnglish
Pages (from-to)545-548
Number of pages4
JournalCereal Research Communications
Issue numberSUPPL. 6
Publication statusPublished - 2008


  • Arabidopsis
  • Biotransformation
  • Yeast bioassay
  • Zearalenone


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