Absence of far-red emission band in aggregated core antenna complexes

Anjue Mane Ara, Mohammad Kawsar Ahmed, Sandrine D'Haene, Henny van Roon, Cristian Ilioaia, Rienk van Grondelle, Md Wahadoszamen*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Reported herein is a Stark fluorescence spectroscopy study performed on photosystem II core antenna complexes CP43 and CP47 in their native and aggregated states. The systematic mathematical modeling of the Stark fluorescence spectra with the aid of conventional Liptay formalism revealed that induction of aggregation in both the core antenna complexes via detergent removal results in a single quenched species characterized by a remarkably broad and inhomogenously broadened emission lineshape peaking around 700 nm. The quenched species possesses a fairly large magnitude of charge-transfer character. From the analogy with the results from aggregated peripheral antenna complexes, the quenched species is thought to originate from the enhanced chlorophyll-chlorophyll interaction due to aggregation. However, in contrast, aggregation of both core antenna complexes did not produce a far-red emission band at ∼730 nm, which was identified in most of the aggregated peripheral antenna complexes. The 730-nm emission band of the aggregated peripheral antenna complexes was attributed to the enhanced chlorophyll-carotenoid (lutein1) interaction in the terminal emitter locus. Therefore, it is very likely that the no occurrence of the far-red band in the aggregated core antenna complexes is directly related to the absence of lutein1 in their structures. The absence of the far-red band also suggests the possibility that aggregation-induced conformational change of the core antenna complexes does not yield a chlorophyll-carotenoid interaction associated energy dissipation channel.

Original languageEnglish
Pages (from-to)1680-1691
Number of pages12
JournalBiophysical Journal
Volume120
Issue number9
Early online date4 Mar 2021
DOIs
Publication statusPublished - 4 May 2021

Bibliographical note

Funding Information:
The authors thankfully acknowledge the contribution of Jos Thieme for his technical assistance.Md.W., A.M.A., C.I., and R.v.G. were supported by the Vrije University Amsterdam , the Laserlab-Europe Consortium and the advanced investigator grant ( 267333 , PHOTPROT) from the European Research Council . Md.W., C.I., and R.v.G. were supported further by the TOP Grant ( 700.58.305 ) from the Foundation of Chemical Sciences , part of NWO. C.I. was awarded an EU FP7 Marie Curie Reintegration Grant ( ERG 224796 ).

Publisher Copyright:
© 2021 Biophysical Society

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

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