ACE inhibition modifies exercise-induced pro-angiogenic and mitochondrial gene transcript expression

S. van Ginkel, S. Ruoss, P. Valdivieso, H. Degens, S. Waldron, Arnold de Haan, M. Flück*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Skeletal muscle responds to endurance exercise with an improvement of biochemical pathways that support substrate supply and oxygen-dependent metabolism. This is reflected by enhanced expression of associated factors after exercise and is specifically modulated by tissue perfusion and oxygenation. We hypothesized that transcript expression of pro-angiogenic factors (VEGF, tenascin-C, Angpt1, Angpt1R) and oxygen metabolism (COX4I1, COX4I2, HIF-1α) in human muscle after an endurance stimulus depends on vasoconstriction, and would be modulated through angiotensin-converting enzyme inhibition by intake of lisinopril. Fourteen non-specifically trained, male Caucasians subjects, carried out a single bout of standardized one-legged bicycle exercise. Seven of the participants consumed lisinopril in the 3 days before exercise. Biopsies were collected pre- and 3 h post-exercise from the m. vastus lateralis. COX4I1 (P = 0.03), COX4I2 (P = 0.04) mRNA and HIF-1α (P = 0.05) mRNA and protein levels (P = 0.01) showed an exercise-induced increase in the group not consuming the ACE inhibitor. Conversely, there was a specific exercise-induced increase in VEGF transcript (P = 0.04) and protein levels (P = 0.03) and a trend for increased tenascin-c transcript levels (P = 0.09) for subjects consuming lisinopril. The observations indicate that exercise-induced expression of transcripts involved in angiogenesis and mitochondrial energy metabolism are to some extent regulated via a hypoxia-related ACE-dependent mechanism.

Original languageEnglish
Pages (from-to)1180-1187
Number of pages8
JournalScandinavian Journal of Medicine and Science in Sports
Volume26
Issue number10
DOIs
Publication statusPublished - 1 Oct 2016

Keywords

  • angiotensin
  • exercise
  • gene
  • hypertension
  • hypoxia
  • lisinopril
  • Muscle
  • perfusion

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