Abstract
Water concentration affects both the enantioselectivity and activity of enzymes in dry organic media. Its influence has been investigated using the hydrocyanation of benzaldehyde catalyzed by hydroxynitrile lyase cross-linked enzyme aggregate (A/eHNL-CLEA) as a model reaction. The enzyme displayed higher enantioselectivity at higher water concentration, thus suggesting a positive effect of enzyme flexibility on selectivity. The activity increased on reducing the solvent water content, but drastic dehydration of the enzyme resulted in a reversible loss of activity. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Weinheim.
Original language | English |
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Pages (from-to) | 7596-7604 |
Number of pages | 9 |
Journal | Chemistry: A European Journal |
Volume | 16 |
Issue number | 25 |
DOIs | |
Publication status | Published - 2010 |