Activity and Enantioselectivity of the Hydroxynitrile Lyase MeHNL in Dry Organic Solvents

M. Paravidino, M.J. Sorgedrager, R.V.A. Orru, U. Hanefeld

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Water concentration affects both the enantioselectivity and activity of enzymes in dry organic media. Its influence has been investigated using the hydrocyanation of benzaldehyde catalyzed by hydroxynitrile lyase cross-linked enzyme aggregate (A/eHNL-CLEA) as a model reaction. The enzyme displayed higher enantioselectivity at higher water concentration, thus suggesting a positive effect of enzyme flexibility on selectivity. The activity increased on reducing the solvent water content, but drastic dehydration of the enzyme resulted in a reversible loss of activity. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Weinheim.
Original languageEnglish
Pages (from-to)7596-7604
Number of pages9
JournalChemistry: A European Journal
Volume16
Issue number25
DOIs
Publication statusPublished - 2010

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