Water concentration affects both the enantioselectivity and activity of enzymes in dry organic media. Its influence has been investigated using the hydrocyanation of benzaldehyde catalyzed by hydroxynitrile lyase cross-linked enzyme aggregate (A/eHNL-CLEA) as a model reaction. The enzyme displayed higher enantioselectivity at higher water concentration, thus suggesting a positive effect of enzyme flexibility on selectivity. The activity increased on reducing the solvent water content, but drastic dehydration of the enzyme resulted in a reversible loss of activity. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Weinheim.
Paravidino, M., Sorgedrager, M. J., Orru, R. V. A., & Hanefeld, U. (2010). Activity and Enantioselectivity of the Hydroxynitrile Lyase MeHNL in Dry Organic Solvents. Chemistry: A European Journal, 16(25), 7596-7604. https://doi.org/10.1002/chem.201000487