Abstract
Water concentration affects both the enantioselectivity and activity of enzymes in dry organic media. Its influence has been investigated using the hydrocyanation of benzaldehyde catalyzed by hydroxynitrile lyase cross-linked enzyme aggregate (A/eHNL-CLEA) as a model reaction. The enzyme displayed higher enantioselectivity at higher water concentration, thus suggesting a positive effect of enzyme flexibility on selectivity. The activity increased on reducing the solvent water content, but drastic dehydration of the enzyme resulted in a reversible loss of activity. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Weinheim.
| Original language | English |
|---|---|
| Pages (from-to) | 7596-7604 |
| Number of pages | 9 |
| Journal | Chemistry: A European Journal |
| Volume | 16 |
| Issue number | 25 |
| DOIs | |
| Publication status | Published - 2010 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 6 Clean Water and Sanitation
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