Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples

Tianlong Man, Yuhong Wan, Wujuan Yan, Xiu Hong Wang, Erwin J.G. Peterman, Dayong Wang

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system. We show proof-of-principle experiments on a tissue phantom containing fluorescence particles. Furthermore, we present three-dimensional reconstructions of actin-labeled MCF7 breast cancer cells, showing improved resolution after the correction of aberrations. Both experiments demonstrate the validity of our method and show the great potential of non-scanning adaptive three-dimensional microscopy in imaging biological samples with improved resolution and signal-to-noise ratio.

Original languageEnglish
Article number#326210
Pages (from-to)2614-2626
Number of pages13
JournalBiomedical Optics Express
Volume9
Issue number6
Early online date10 May 2018
DOIs
Publication statusPublished - 1 Jun 2018

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Holography
Three-Dimensional Imaging
adaptive optics
holography
aberration
interference
Signal-To-Noise Ratio
Fluorescence Microscopy
microscopy
Actins
Microscopy
fluorescence
Fluorescence
Breast Neoplasms
breast
low speed
acquisition
inhomogeneity
signal to noise ratios
cancer

Cite this

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title = "Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples",
abstract = "Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system. We show proof-of-principle experiments on a tissue phantom containing fluorescence particles. Furthermore, we present three-dimensional reconstructions of actin-labeled MCF7 breast cancer cells, showing improved resolution after the correction of aberrations. Both experiments demonstrate the validity of our method and show the great potential of non-scanning adaptive three-dimensional microscopy in imaging biological samples with improved resolution and signal-to-noise ratio.",
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Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples. / Man, Tianlong; Wan, Yuhong; Yan, Wujuan; Wang, Xiu Hong; Peterman, Erwin J.G.; Wang, Dayong.

In: Biomedical Optics Express, Vol. 9, No. 6, #326210, 01.06.2018, p. 2614-2626.

Research output: Contribution to JournalArticleAcademicpeer-review

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