Abstract
Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system. We show proof-of-principle experiments on a tissue phantom containing fluorescence particles. Furthermore, we present three-dimensional reconstructions of actin-labeled MCF7 breast cancer cells, showing improved resolution after the correction of aberrations. Both experiments demonstrate the validity of our method and show the great potential of non-scanning adaptive three-dimensional microscopy in imaging biological samples with improved resolution and signal-to-noise ratio.
| Original language | English |
|---|---|
| Article number | #326210 |
| Pages (from-to) | 2614-2626 |
| Number of pages | 13 |
| Journal | Biomedical Optics Express |
| Volume | 9 |
| Issue number | 6 |
| Early online date | 10 May 2018 |
| DOIs | |
| Publication status | Published - 1 Jun 2018 |
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Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples. / Man, Tianlong; Wan, Yuhong; Yan, Wujuan; Wang, Xiu Hong; Peterman, Erwin J.G.; Wang, Dayong.
In: Biomedical Optics Express, Vol. 9, No. 6, #326210, 01.06.2018, p. 2614-2626.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples
AU - Man, Tianlong
AU - Wan, Yuhong
AU - Yan, Wujuan
AU - Wang, Xiu Hong
AU - Peterman, Erwin J.G.
AU - Wang, Dayong
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system. We show proof-of-principle experiments on a tissue phantom containing fluorescence particles. Furthermore, we present three-dimensional reconstructions of actin-labeled MCF7 breast cancer cells, showing improved resolution after the correction of aberrations. Both experiments demonstrate the validity of our method and show the great potential of non-scanning adaptive three-dimensional microscopy in imaging biological samples with improved resolution and signal-to-noise ratio.
AB - Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system. We show proof-of-principle experiments on a tissue phantom containing fluorescence particles. Furthermore, we present three-dimensional reconstructions of actin-labeled MCF7 breast cancer cells, showing improved resolution after the correction of aberrations. Both experiments demonstrate the validity of our method and show the great potential of non-scanning adaptive three-dimensional microscopy in imaging biological samples with improved resolution and signal-to-noise ratio.
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UR - http://www.scopus.com/inward/citedby.url?scp=85048192226&partnerID=8YFLogxK
U2 - 10.1364/BOE.9.002614
DO - 10.1364/BOE.9.002614
M3 - Article
VL - 9
SP - 2614
EP - 2626
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 6
M1 - #326210
ER -