Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples

Tianlong Man, Yuhong Wan, Wujuan Yan, Xiu Hong Wang, Erwin J.G. Peterman, Dayong Wang

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system. We show proof-of-principle experiments on a tissue phantom containing fluorescence particles. Furthermore, we present three-dimensional reconstructions of actin-labeled MCF7 breast cancer cells, showing improved resolution after the correction of aberrations. Both experiments demonstrate the validity of our method and show the great potential of non-scanning adaptive three-dimensional microscopy in imaging biological samples with improved resolution and signal-to-noise ratio.

Original languageEnglish
Article number#326210
Pages (from-to)2614-2626
Number of pages13
JournalBiomedical Optics Express
Volume9
Issue number6
Early online date10 May 2018
DOIs
Publication statusPublished - 1 Jun 2018

Fingerprint

Dive into the research topics of 'Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples'. Together they form a unique fingerprint.

Cite this