Additive genetic variation of transcriptional regulation: metallothionein expression in the soil insect Orchesella cincta.

T.F.M. Roelofs, L. Overhein, M.E. de Boer, T.K.S. Janssens, N.M. van Straalen

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Field-selected metal tolerance in Orchesella cincta is correlated with overexpression of the single copy cadmium (Cd) inducible metallothionein (mt). Previously, we have demonstrated large phenotypic variation in mt gene expression, and a higher frequency of high-expression phenotypes in a tolerant population. Here, we describe midparent-offspring regression analysis of mt gene expression in a laboratory culture originating from a noncontaminated natural population. Families were either not exposed (n = 47) or exposed to 0.5 μmol Cd per gram dry food (n = 46). Mean mt gene expressions normalized to 28S rRNA and β-actin RNA were generated using real-time RT-PCR applied to parents and offspring RNA and subjected to regression analysis. A significant heritability (h
    Original languageEnglish
    Pages (from-to)85-92
    JournalHeredity
    Volume96
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Dive into the research topics of 'Additive genetic variation of transcriptional regulation: metallothionein expression in the soil insect Orchesella cincta.'. Together they form a unique fingerprint.

    Cite this