Adsorption of bovine alpha-lactalbumin on suspended solid nanospheres and its subsequent displacement studied by NMR spectroscopy.

M.F.M. Engel; A.J.W.G. Visser; C.P.M. Van Mierlo

doi:10.1021/la049834b

Adsorption of bovine alpha-lactalbumin on suspended solid nanospheres and its subsequent displacement studied by NMR spectroscopy.

M.F.M. Engel, A.J.W.G. Visser, C.P.M. Van Mierlo

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The adsorption of bovine α-lactalbumin (BLA) on solid polystyrene nanospheres was analyzed in solution using nuclear magnetic resonance (NMR) spectroscopy. It was shown that the amide protons of displaced BLA resonate at identical positions as the corresponding protons of native BLA. BLA refolded to the native state after its displacement from the nanospheres. The results show that the requirements are fulfilled for the study by H/D exchange and NMR spectroscopy of BLA adsorbed on polystyrene nanospheres.

Original language	English
Pages (from-to)	5530-5538
Number of pages	9
Journal	Langmuir
Volume	20
DOIs	https://doi.org/10.1021/la049834b
Publication status	Published - 2004

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title = "Adsorption of bovine alpha-lactalbumin on suspended solid nanospheres and its subsequent displacement studied by NMR spectroscopy.",

abstract = "The adsorption of bovine α-lactalbumin (BLA) on solid polystyrene nanospheres was analyzed in solution using nuclear magnetic resonance (NMR) spectroscopy. It was shown that the amide protons of displaced BLA resonate at identical positions as the corresponding protons of native BLA. BLA refolded to the native state after its displacement from the nanospheres. The results show that the requirements are fulfilled for the study by H/D exchange and NMR spectroscopy of BLA adsorbed on polystyrene nanospheres.",

author = "M.F.M. Engel and A.J.W.G. Visser and {Van Mierlo}, C.P.M.",

year = "2004",

doi = "10.1021/la049834b",

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journal = "Langmuir",

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AU - Engel, M.F.M.

AU - Visser, A.J.W.G.

AU - Van Mierlo, C.P.M.

PY - 2004

Y1 - 2004

N2 - The adsorption of bovine α-lactalbumin (BLA) on solid polystyrene nanospheres was analyzed in solution using nuclear magnetic resonance (NMR) spectroscopy. It was shown that the amide protons of displaced BLA resonate at identical positions as the corresponding protons of native BLA. BLA refolded to the native state after its displacement from the nanospheres. The results show that the requirements are fulfilled for the study by H/D exchange and NMR spectroscopy of BLA adsorbed on polystyrene nanospheres.

AB - The adsorption of bovine α-lactalbumin (BLA) on solid polystyrene nanospheres was analyzed in solution using nuclear magnetic resonance (NMR) spectroscopy. It was shown that the amide protons of displaced BLA resonate at identical positions as the corresponding protons of native BLA. BLA refolded to the native state after its displacement from the nanospheres. The results show that the requirements are fulfilled for the study by H/D exchange and NMR spectroscopy of BLA adsorbed on polystyrene nanospheres.

U2 - 10.1021/la049834b

DO - 10.1021/la049834b

M3 - Article

SN - 0743-7463

VL - 20

SP - 5530

EP - 5538

JO - Langmuir

JF - Langmuir

ER -

Adsorption of bovine alpha-lactalbumin on suspended solid nanospheres and its subsequent displacement studied by NMR spectroscopy.

Abstract

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