Adsorption of bovine alpha-lactalbumin on suspended solid nanospheres and its subsequent displacement studied by NMR spectroscopy.

M.F.M. Engel, A.J.W.G. Visser, C.P.M. Van Mierlo

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The adsorption of bovine α-lactalbumin (BLA) on solid polystyrene nanospheres was analyzed in solution using nuclear magnetic resonance (NMR) spectroscopy. It was shown that the amide protons of displaced BLA resonate at identical positions as the corresponding protons of native BLA. BLA refolded to the native state after its displacement from the nanospheres. The results show that the requirements are fulfilled for the study by H/D exchange and NMR spectroscopy of BLA adsorbed on polystyrene nanospheres.
Original languageEnglish
Pages (from-to)5530-5538
Number of pages9
JournalLangmuir
Volume20
DOIs
Publication statusPublished - 2004

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