Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA

N. Laurens, R.P.C. Driessen, I. Heller, D. Vorselen, M.C. Noom, F.J.H. Hol, M.F. White, R.T. Dame, G.J.L. Wuite

Research output: Contribution to JournalArticleAcademicpeer-review

196 Downloads (Pure)

Abstract

Architectural proteins have an important role in shaping the genome and act as global regulators of gene expression. How these proteins jointly modulate genome plasticity is largely unknown. In archaea, one of the most abundant proteins, Alba, is considered to have a key role in organizing the genome. Here we characterize the multimodal architectural properties and interplay of the Alba1 and Alba2 proteins using single-molecule imaging and manipulation techniques. We demonstrate that the two paralogues can bridge and rigidify DNA and that the interplay between the two proteins influences the balance between these effects. Our data yield a structural model that explains the multimodal behaviour of Alba proteins and its impact on genome folding. © 2012 Macmillan Publishers Limited. All rights reserved.
Original languageEnglish
Article number1328
JournalNature Communications
Volume3
DOIs
Publication statusPublished - 2012

Fingerprint

Dive into the research topics of 'Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA'. Together they form a unique fingerprint.

Cite this