Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells.

A.-K. Gustavsson, D.D. van Niekerk, C.B. Adiels, B.W. Kooi, M. Goksor, J.L. Snoep

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Oscillations are widely distributed in nature and synchronization of oscillators has been described at the cellular level (e.g. heart cells) and at the population level (e.g. fireflies). Yeast glycolysis is the best known oscillatory system, although it has been studied almost exclusively at the population level (i.e. limited to observations of average behaviour in synchronized cultures). We studied individual yeast cells that were positioned with optical tweezers in a microfluidic chamber to determine the precise conditions for autonomous glycolytic oscillations. Hopf bifurcation points were determined experimentally in individual cells as a function of glucose and cyanide concentrations. The experiments were analyzed in a detailed mathematical model and could be interpreted in terms of an oscillatory manifold in a three-dimensional state-space; crossing the boundaries of the manifold coincides with the onset of oscillations and positioning along the longitudinal axis of the volume sets the period. The oscillatory manifold could be approximated by allosteric control values of phosphofructokinase for ATP and AMP. © 2014 FEBS.
Original languageEnglish
Pages (from-to)2784-2793
Number of pages10
JournalThe FEBS Journal
Volume281
Issue number12
DOIs
Publication statusPublished - 2014

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Allosteric Regulation
Phosphofructokinases
Yeast
Yeasts
Cells
Optical tweezers
Hopf bifurcation
Cyanides
Adenosine Monophosphate
Fireflies
Cell culture
Microfluidics
Optical Tweezers
Synchronization
Adenosine Triphosphate
Glycolysis
Mathematical models
Glucose
Population
Theoretical Models

Cite this

Gustavsson, A.-K. ; van Niekerk, D.D. ; Adiels, C.B. ; Kooi, B.W. ; Goksor, M. ; Snoep, J.L. / Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells. In: The FEBS Journal. 2014 ; Vol. 281, No. 12. pp. 2784-2793.
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Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells. / Gustavsson, A.-K.; van Niekerk, D.D.; Adiels, C.B.; Kooi, B.W.; Goksor, M.; Snoep, J.L.

In: The FEBS Journal, Vol. 281, No. 12, 2014, p. 2784-2793.

Research output: Contribution to JournalArticleAcademicpeer-review

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AB - Oscillations are widely distributed in nature and synchronization of oscillators has been described at the cellular level (e.g. heart cells) and at the population level (e.g. fireflies). Yeast glycolysis is the best known oscillatory system, although it has been studied almost exclusively at the population level (i.e. limited to observations of average behaviour in synchronized cultures). We studied individual yeast cells that were positioned with optical tweezers in a microfluidic chamber to determine the precise conditions for autonomous glycolytic oscillations. Hopf bifurcation points were determined experimentally in individual cells as a function of glucose and cyanide concentrations. The experiments were analyzed in a detailed mathematical model and could be interpreted in terms of an oscillatory manifold in a three-dimensional state-space; crossing the boundaries of the manifold coincides with the onset of oscillations and positioning along the longitudinal axis of the volume sets the period. The oscillatory manifold could be approximated by allosteric control values of phosphofructokinase for ATP and AMP. © 2014 FEBS.

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