Abstract
The human alpha-Synuclein (αS) protein is of significant interest because of its association with Parkinson's disease and related neurodegenerative disorders. The intrinsically disordered protein (140 amino acids) is characterized by the absence of a well-defined structure in solution. It displays remarkable conformational flexibility upon macromolecular interactions, and can associate with mitochondrial membranes. Site-directed spin-labeling in combination with electron paramagnetic resonance spectroscopy enabled us to study the local binding properties of αS on artificial membranes (mimicking the inner and outer mitochondrial membranes), and to evaluate the importance of cardiolipin in this interaction. With pulsed, two-frequency, double-electron electron paramagnetic resonance (DEER) approaches, we examined, to the best of our knowledge for the first time, the conformation of αS bound to isolated mitochondria.
Original language | English |
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Pages (from-to) | 2499-502 |
Number of pages | 4 |
Journal | ChemBioChem |
Volume | 15 |
Issue number | 17 |
DOIs | |
Publication status | Published - 24 Nov 2014 |
Keywords
- Binding Sites
- HEK293 Cells
- Humans
- Mitochondria
- Mitochondrial Membranes
- Protein Conformation
- alpha-Synuclein
- Journal Article
- Research Support, Non-U.S. Gov't