Alteration of extracellular matrix proteins in atrophic periodontal ligament of hypofunctional rat molars

Daneeya Na Nan, Vincent Everts, Joao N. Ferreira, Vorapat Trachoo, Thanaphum Osathanon, Nuttha Klincumhom*, Prasit Pavasant

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Objectives: The aim of this study was to investigate the effect of mechanical force on possible dynamic changes of the matrix proteins deposition in the PDL upon in vitro mechanical and in vivo occlusal forces in a rat model with hypofunctional conditions. Materials and methods: Intermittent compressive force (ICF) and shear force (SF) were applied to human periodontal ligament stem cells (PDLSCs). Protein expression of collagen I and POSTN was analyzed by western blot technique. To establish an in vivo model, rat maxillary molars were extracted to facilitate hypofunction of the periodontal ligament (PDL) tissue of the opposing mandibular molar. The mandibles were collected after 4-, 8-, and 12-weeks post-extraction and used for micro-CT and immunohistochemical analysis. Results: ICF and SF increased the synthesis of POSTN by human PDLSCs. Histological changes in the hypofunctional teeth revealed a narrowing of the PDL space, along with a decreased amount of collagen I, POSTN, and laminin in perivascular structures compared to the functional contralateral molars. Conclusion: Our results revealed that loss of occlusal force disrupts deposition of some major matrix proteins in the PDL, underscoring the relevance of mechanical forces in maintaining periodontal tissue homeostasis by modulating ECM composition.

Original languageEnglish
Article number31
Pages (from-to)1-7
Number of pages7
JournalBDJ Open
Volume9
DOIs
Publication statusPublished - 18 Jul 2023

Bibliographical note

Funding Information:
This work was supported by Research Exchange Grant, Faculty of Dentistry, Chulalongkorn University (to DN); Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University and Chulalongkorn University and Faculty Research Fund, Faculty of Dentistry, Chulalongkorn University (to NK); The NSRF via The Program Management Unit for Human Resources & Institutional Development, Research and Innovation (B16F640118 to TO); Thailand Science Research and Innovation (RTA6180001 to PP).

Funding Information:
The authors thank all staff in the Center of Excellence in Regenerative Dentistry and the Department of Surgery, Chulalongkorn University, for the human specimens.

Publisher Copyright:
© 2023, The Author(s).

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