Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.

A. Bonifacio, A.R. Groenhof, P.H.J. Keizers, C. de Graaf, J.N.M. Commandeur, N.P.E. Vermeulen, A.W. Ehlers, K. Lammertsma, C. Gooijer, G. van der Zwan

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Abstract

Cytochrome P450 2D6 (CYP2D6) is one of the most important cytochromes P450 in humans. Resonance Raman data from the T309V mutant of CYP2D6 show that the substitution of the conserved I-helix threonine situated in the enzyme's active site perturbs the heme spin equilibrium in favor of the six-coordinated low-spin species. A mechanistic hypothesis is introduced to explain the experimental observations, and its compatibility with the available structural and spectroscopic data is tested using quantum-mechanical density functional theory calculations on active-site models for both the CYP2D6 wild type and the T309V mutant. © 2007 SBIC.
Original languageEnglish
Pages (from-to)645-654
JournalJournal of Biological Inorganic Chemistry - JBIC
Volume12
Issue number5
DOIs
Publication statusPublished - 2007

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