Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.

A. Bonifacio; A.R. Groenhof; P.H.J. Keizers; C. de Graaf; J.N.M. Commandeur; N.P.E. Vermeulen; A.W. Ehlers; K. Lammertsma; C. Gooijer; G. van der Zwan

doi:10.1007/s00775-007-0210-5

Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.

A. Bonifacio, A.R. Groenhof, P.H.J. Keizers, C. de Graaf, J.N.M. Commandeur, N.P.E. Vermeulen, A.W. Ehlers, K. Lammertsma, C. Gooijer, G. van der Zwan

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Abstract

Cytochrome P450 2D6 (CYP2D6) is one of the most important cytochromes P450 in humans. Resonance Raman data from the T309V mutant of CYP2D6 show that the substitution of the conserved I-helix threonine situated in the enzyme's active site perturbs the heme spin equilibrium in favor of the six-coordinated low-spin species. A mechanistic hypothesis is introduced to explain the experimental observations, and its compatibility with the available structural and spectroscopic data is tested using quantum-mechanical density functional theory calculations on active-site models for both the CYP2D6 wild type and the T309V mutant. © 2007 SBIC.

Original language	English
Pages (from-to)	645-654
Journal	Journal of Biological Inorganic Chemistry - JBIC
Volume	12
Issue number	5
DOIs	https://doi.org/10.1007/s00775-007-0210-5
Publication status	Published - 2007

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Bonifacio, A., Groenhof, A. R., Keizers, P. H. J., de Graaf, C., Commandeur, J. N. M., Vermeulen, N. P. E., Ehlers, A. W., Lammertsma, K., Gooijer, C., & van der Zwan, G. (2007). Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study. Journal of Biological Inorganic Chemistry - JBIC, 12(5), 645-654. https://doi.org/10.1007/s00775-007-0210-5

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title = "Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.",

abstract = "Cytochrome P450 2D6 (CYP2D6) is one of the most important cytochromes P450 in humans. Resonance Raman data from the T309V mutant of CYP2D6 show that the substitution of the conserved I-helix threonine situated in the enzyme's active site perturbs the heme spin equilibrium in favor of the six-coordinated low-spin species. A mechanistic hypothesis is introduced to explain the experimental observations, and its compatibility with the available structural and spectroscopic data is tested using quantum-mechanical density functional theory calculations on active-site models for both the CYP2D6 wild type and the T309V mutant. {\textcopyright} 2007 SBIC.",

author = "A. Bonifacio and A.R. Groenhof and P.H.J. Keizers and {de Graaf}, C. and J.N.M. Commandeur and N.P.E. Vermeulen and A.W. Ehlers and K. Lammertsma and C. Gooijer and {van der Zwan}, G.",

year = "2007",

doi = "10.1007/s00775-007-0210-5",

language = "English",

volume = "12",

pages = "645--654",

journal = "Journal of Biological Inorganic Chemistry - JBIC",

issn = "0949-8257",

publisher = "Springer Verlag",

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Bonifacio, A, Groenhof, AR, Keizers, PHJ, de Graaf, C, Commandeur, JNM, Vermeulen, NPE, Ehlers, AW, Lammertsma, K, Gooijer, C & van der Zwan, G 2007, 'Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.', Journal of Biological Inorganic Chemistry - JBIC, vol. 12, no. 5, pp. 645-654. https://doi.org/10.1007/s00775-007-0210-5

TY - JOUR

T1 - Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.

AU - Bonifacio, A.

AU - Groenhof, A.R.

AU - Keizers, P.H.J.

AU - de Graaf, C.

AU - Commandeur, J.N.M.

AU - Vermeulen, N.P.E.

AU - Ehlers, A.W.

AU - Lammertsma, K.

AU - Gooijer, C.

AU - van der Zwan, G.

PY - 2007

Y1 - 2007

N2 - Cytochrome P450 2D6 (CYP2D6) is one of the most important cytochromes P450 in humans. Resonance Raman data from the T309V mutant of CYP2D6 show that the substitution of the conserved I-helix threonine situated in the enzyme's active site perturbs the heme spin equilibrium in favor of the six-coordinated low-spin species. A mechanistic hypothesis is introduced to explain the experimental observations, and its compatibility with the available structural and spectroscopic data is tested using quantum-mechanical density functional theory calculations on active-site models for both the CYP2D6 wild type and the T309V mutant. © 2007 SBIC.

AB - Cytochrome P450 2D6 (CYP2D6) is one of the most important cytochromes P450 in humans. Resonance Raman data from the T309V mutant of CYP2D6 show that the substitution of the conserved I-helix threonine situated in the enzyme's active site perturbs the heme spin equilibrium in favor of the six-coordinated low-spin species. A mechanistic hypothesis is introduced to explain the experimental observations, and its compatibility with the available structural and spectroscopic data is tested using quantum-mechanical density functional theory calculations on active-site models for both the CYP2D6 wild type and the T309V mutant. © 2007 SBIC.

U2 - 10.1007/s00775-007-0210-5

DO - 10.1007/s00775-007-0210-5

M3 - Article

SN - 0949-8257

VL - 12

SP - 645

EP - 654

JO - Journal of Biological Inorganic Chemistry - JBIC

JF - Journal of Biological Inorganic Chemistry - JBIC

IS - 5

ER -

Altered spin state equilibrium in the T309V mutant of cytochrome P450 2D6: a spectroscopic and computational study.

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