An excess of glycolytic enzymes under glucose-limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availability

Pranas Grigaitis; Bas Teusink

doi:10.1002/1873-3468.14484

An excess of glycolytic enzymes under glucose-limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availability

Pranas Grigaitis^*, Bas Teusink

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

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Abstract

Microorganisms, including the budding yeast Saccharomyces cerevisiae, express glycolytic proteins to a maximal capacity that (largely) exceeds the actual flux through the enzymes, especially at low growth rates. An open question is if this apparent expression level is really an overcapacity, or maintains the (optimal) enzyme capacity needed to carry flux at (very) low substrate availability. Here, we use computational modelling to suggest that yeast maintains a genuine excess of glycolytic enzymes at low specific growth rates. During fast fermentative growth at high glucose levels, the observed expression of the glycolytic enzymes matched the predicted optimal levels. We suggest that the excess glycolytic capacity at low glucose levels is a preparatory strategy in the adaptation to sugar fluctuations in the environment.

Original language	English
Pages (from-to)	3203-3210
Number of pages	8
Journal	FEBS Letters
Volume	596
Issue number	24
Early online date	25 Aug 2022
DOIs	https://doi.org/10.1002/1873-3468.14484
Publication status	Published - Dec 2022

Bibliographical note

Funding Information:
We thank Ursula Kummer for discussions and feedback on the results, and Elad Noor and Wolfram Liebermeister for the discussions regarding the ECM method. The authors acknowledge the funding by Marie Skłodowska‐Curie Actions ITN ‘SynCrop’ (grant agreement No 764591) and NWO (NWO ERA‐IB‐2, project No 053.80.722).

Publisher Copyright:
© 2022 Federation of European Biochemical Societies.

Funding

We thank Ursula Kummer for discussions and feedback on the results, and Elad Noor and Wolfram Liebermeister for the discussions regarding the ECM method. The authors acknowledge the funding by Marie Skłodowska‐Curie Actions ITN ‘SynCrop’ (grant agreement No 764591) and NWO (NWO ERA‐IB‐2, project No 053.80.722).

Keywords

budding yeast
enzyme capacity
glycolysis
resource allocation

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10.1002/1873-3468.14484

An excess of glycolytic enzymes under glucose-limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availabilityFinal published version, 702 KBLicence: Article 25fa Dutch Copyright Act

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title = "An excess of glycolytic enzymes under glucose-limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availability",

abstract = "Microorganisms, including the budding yeast Saccharomyces cerevisiae, express glycolytic proteins to a maximal capacity that (largely) exceeds the actual flux through the enzymes, especially at low growth rates. An open question is if this apparent expression level is really an overcapacity, or maintains the (optimal) enzyme capacity needed to carry flux at (very) low substrate availability. Here, we use computational modelling to suggest that yeast maintains a genuine excess of glycolytic enzymes at low specific growth rates. During fast fermentative growth at high glucose levels, the observed expression of the glycolytic enzymes matched the predicted optimal levels. We suggest that the excess glycolytic capacity at low glucose levels is a preparatory strategy in the adaptation to sugar fluctuations in the environment.",

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AU - Teusink, Bas

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N2 - Microorganisms, including the budding yeast Saccharomyces cerevisiae, express glycolytic proteins to a maximal capacity that (largely) exceeds the actual flux through the enzymes, especially at low growth rates. An open question is if this apparent expression level is really an overcapacity, or maintains the (optimal) enzyme capacity needed to carry flux at (very) low substrate availability. Here, we use computational modelling to suggest that yeast maintains a genuine excess of glycolytic enzymes at low specific growth rates. During fast fermentative growth at high glucose levels, the observed expression of the glycolytic enzymes matched the predicted optimal levels. We suggest that the excess glycolytic capacity at low glucose levels is a preparatory strategy in the adaptation to sugar fluctuations in the environment.

AB - Microorganisms, including the budding yeast Saccharomyces cerevisiae, express glycolytic proteins to a maximal capacity that (largely) exceeds the actual flux through the enzymes, especially at low growth rates. An open question is if this apparent expression level is really an overcapacity, or maintains the (optimal) enzyme capacity needed to carry flux at (very) low substrate availability. Here, we use computational modelling to suggest that yeast maintains a genuine excess of glycolytic enzymes at low specific growth rates. During fast fermentative growth at high glucose levels, the observed expression of the glycolytic enzymes matched the predicted optimal levels. We suggest that the excess glycolytic capacity at low glucose levels is a preparatory strategy in the adaptation to sugar fluctuations in the environment.

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KW - enzyme capacity

KW - glycolysis

KW - resource allocation

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An excess of glycolytic enzymes under glucose-limited conditions may enable Saccharomyces cerevisiae to adapt to nutrient availability

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