Analysis of glutathione adducts of patulin by means of liquid chromatography (HPLC) with biochemical detection (BCD) and electrospray ionization tandem mass spectrometry (ESI-MS/MS)

N.H. Schebb, H. Faber, R. Maul, F. Heus, J. Kool, H. Irth, U. Karst

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A novel method for the identification of glutathione/electrophile adducts that are inhibiting glutathione-S-transferase (GST) activity was developed and applied for the analysis of the mycotoxin patulin. The method is based on high-performance liquid chromatography (HPLC) coupled to a continuous-flow enzyme reactor serving as biochemical detector (BCD) in parallel to electrospray mass spectrometric detection (ESI-MS). This HPLC-BCD technique combines a separation step and the detection of the inhibition and is therefore ideally suited for the analysis of the activity of single patulin/glutathione adducts within a complex mixture of adducts. Two out of at least 15 detected patulin-glutathione adducts showed strong GST inhibition. In ESI-MS, the inhibitory active adducts were characterized by [M + H]
Original languageEnglish
Pages (from-to)1361-73
JournalAnalytical and Bioanalytical Chemistry
Volume394
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Dive into the research topics of 'Analysis of glutathione adducts of patulin by means of liquid chromatography (HPLC) with biochemical detection (BCD) and electrospray ionization tandem mass spectrometry (ESI-MS/MS)'. Together they form a unique fingerprint.

Cite this