Analytics for bioactivity profiling of complex mixtures with a focus on venoms

Marija Mladic, Wilfried M.A. Niessen, Govert W. Somsen, Jeroen Kool*

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

This chapter introduces bioactivity and bioaffinity terms in relation to mixture profiling and gives the significance of bioactivity and/or bioaffinity profiling of biologically active mixtures in general, and for bioactive mixtures in drug discovery research in particular. Further, the chapter gives an overview of the common and less common analytical approaches for bioactivity profiling of bioactive mixtures. Special focus is put on bioassay-guided fractionation as the standard technique employed (in identification and purification of bioactive molecules from a bioactive mixture), and on state-of-the-art post-column bioactivity profiling approaches, also providing examples and limitations of these analytical methods. On-column and pre-column bioactivity profiling analytics is also discussed. Examples of bioactive molecules identified and purified from different natural products are given with emphasis on molecules isolated from animal venoms. Finally, this chapter briefly discusses the importance of bioactivity profiling of metabolic mixtures in drug discovery.

Original languageEnglish
Title of host publicationSnake and spider toxins
Subtitle of host publicationmethods and protocols
PublisherHumana Press Inc.
Chapter2
Pages27-49
Number of pages23
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology
Volume2068
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Analytical approaches
  • Bioactivity profiling
  • Drug discovery
  • Metabolic mixtures
  • Natural products
  • Venoms

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