Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

Y. Rombouts; E. Ewing; L.A. van de Stadt; M.H.J. Selman; L.A. Trouw; A.M. Deelder; T.W.J. Huizinga; M. Wuhrer; D. van Schaardenburg; R.E.M. Toes; H.U. Scherer

doi:10.1136/annrheumdis-2013-203565

Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

Y. Rombouts, E. Ewing, L.A. van de Stadt, M.H.J. Selman, L.A. Trouw, A.M. Deelder, T.W.J. Huizinga, M. Wuhrer, D. van Schaardenburg, R.E.M. Toes, H.U. Scherer

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.

Original language	English
Pages (from-to)	234-241
Journal	Annals of the Rheumatic Diseases
Volume	74
Issue number	1
DOIs	https://doi.org/10.1136/annrheumdis-2013-203565
Publication status	Published - 2015

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Rombouts, Y., Ewing, E., van de Stadt, L. A., Selman, M. H. J., Trouw, L. A., Deelder, A. M., Huizinga, T. W. J., Wuhrer, M., van Schaardenburg, D., Toes, R. E. M., & Scherer, H. U. (2015). Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis. Annals of the Rheumatic Diseases, 74(1), 234-241. https://doi.org/10.1136/annrheumdis-2013-203565

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title = "Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis",

abstract = "Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.",

author = "Y. Rombouts and E. Ewing and {van de Stadt}, L.A. and M.H.J. Selman and L.A. Trouw and A.M. Deelder and T.W.J. Huizinga and M. Wuhrer and {van Schaardenburg}, D. and R.E.M. Toes and H.U. Scherer",

year = "2015",

doi = "10.1136/annrheumdis-2013-203565",

language = "English",

volume = "74",

pages = "234--241",

journal = "Annals of the Rheumatic Diseases",

issn = "0003-4967",

publisher = "Elsevier B.V.",

number = "1",

}

Rombouts, Y, Ewing, E, van de Stadt, LA, Selman, MHJ, Trouw, LA, Deelder, AM, Huizinga, TWJ, Wuhrer, M, van Schaardenburg, D, Toes, REM & Scherer, HU 2015, 'Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis', Annals of the Rheumatic Diseases, vol. 74, no. 1, pp. 234-241. https://doi.org/10.1136/annrheumdis-2013-203565

TY - JOUR

T1 - Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

AU - Rombouts, Y.

AU - Ewing, E.

AU - van de Stadt, L.A.

AU - Selman, M.H.J.

AU - Trouw, L.A.

AU - Deelder, A.M.

AU - Huizinga, T.W.J.

AU - Wuhrer, M.

AU - van Schaardenburg, D.

AU - Toes, R.E.M.

AU - Scherer, H.U.

PY - 2015

Y1 - 2015

N2 - Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.

AB - Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.

U2 - 10.1136/annrheumdis-2013-203565

DO - 10.1136/annrheumdis-2013-203565

M3 - Article

SN - 0003-4967

VL - 74

SP - 234

EP - 241

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

IS - 1

ER -

Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

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