Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

Y. Rombouts; E. Ewing; L.A. van de Stadt; M.H.J. Selman; L.A. Trouw; A.M. Deelder; T.W.J. Huizinga; M. Wuhrer; D. van Schaardenburg; R.E.M. Toes; H.U. Scherer

doi:10.1136/annrheumdis-2013-203565

Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

Y. Rombouts
, E. Ewing
, L.A. van de Stadt
, M.H.J. Selman
, L.A. Trouw
, A.M. Deelder
, T.W.J. Huizinga
, M. Wuhrer
, D. van Schaardenburg
, R.E.M. Toes
, H.U. Scherer

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.

Original language	English
Pages (from-to)	234-241
Journal	Annals of the Rheumatic Diseases
Volume	74
Issue number	1
DOIs	https://doi.org/10.1136/annrheumdis-2013-203565
Publication status	Published - 2015

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SDG 3 Good Health and Well-being

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10.1136/annrheumdis-2013-203565

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Rombouts, Y., Ewing, E., van de Stadt, L. A., Selman, M. H. J., Trouw, L. A., Deelder, A. M., Huizinga, T. W. J., Wuhrer, M., van Schaardenburg, D., Toes, R. E. M., & Scherer, H. U. (2015). Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis. Annals of the Rheumatic Diseases, 74(1), 234-241. https://doi.org/10.1136/annrheumdis-2013-203565

@article{92608695919c445ca67c06d67ae57f42,

title = "Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis",

abstract = "Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.",

author = "Y. Rombouts and E. Ewing and \{van de Stadt\}, L.A. and M.H.J. Selman and L.A. Trouw and A.M. Deelder and T.W.J. Huizinga and M. Wuhrer and \{van Schaardenburg\}, D. and R.E.M. Toes and H.U. Scherer",

year = "2015",

doi = "10.1136/annrheumdis-2013-203565",

language = "English",

volume = "74",

pages = "234--241",

journal = "Annals of the Rheumatic Diseases",

issn = "0003-4967",

publisher = "Elsevier B.V.",

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}

Rombouts, Y, Ewing, E, van de Stadt, LA, Selman, MHJ, Trouw, LA, Deelder, AM, Huizinga, TWJ, Wuhrer, M, van Schaardenburg, D, Toes, REM & Scherer, HU 2015, 'Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis', Annals of the Rheumatic Diseases, vol. 74, no. 1, pp. 234-241. https://doi.org/10.1136/annrheumdis-2013-203565

TY - JOUR

T1 - Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

AU - Rombouts, Y.

AU - Ewing, E.

AU - van de Stadt, L.A.

AU - Selman, M.H.J.

AU - Trouw, L.A.

AU - Deelder, A.M.

AU - Huizinga, T.W.J.

AU - Wuhrer, M.

AU - van Schaardenburg, D.

AU - Toes, R.E.M.

AU - Scherer, H.U.

PY - 2015

Y1 - 2015

N2 - Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.

AB - Methods: Serum samples of patients with ACPA-positive arthralgia (n=183) were collected at baseline and at various time points of follow-up. 105 patients developed arthritis after a median of 12 months (IQR 6-24) and were classified as having either rheumatoid arthritis (RA, n=48) or undifferentiated arthritis (UA, n=57) based on the 1987 American College of Rheumatology (ACR) criteria. ACPA and total serum IgG were isolated by affinity purification and cleaved by trypsin. ACPA-IgG1 Fc-glycopeptides were subsequently analysed by nano-liquid chromatography mass spectrometry and compared to those of total IgG1.

U2 - 10.1136/annrheumdis-2013-203565

DO - 10.1136/annrheumdis-2013-203565

M3 - Article

SN - 0003-4967

VL - 74

SP - 234

EP - 241

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

IS - 1

ER -

Anti-citrullinated protein antibodies acquire a pro-inflammatory Fc glycosylation phenotype prior to the onset of rheumatoid arthritis

Abstract

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